| 疣粒野生稻抗白叶枯病新基因xa32(t)的鉴定及其分子标记定位 |
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| 引用本文： | 阮辉辉,严成其,安德荣,刘仁虎,陈剑平. 疣粒野生稻抗白叶枯病新基因xa32(t)的鉴定及其分子标记定位[J]. 西北农业学报, 2008, 17(6): 170-174 |
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| 作者姓名： | 阮辉辉 严成其 安德荣 刘仁虎 陈剑平 |
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| 作者单位： | [1]西北农林科技大学植物保护学院,陕西杨凌712100 [2]浙江省农业科学院病毒与生物技术研究所,浙江省植物病毒学重点室,农业部动植物病毒学重点实验室,浙江杭州310021 |
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| 基金项目： | Zhejiang Province Foundation Important Item(Z307451) |
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| 摘 要： | xa32（t）是从疣粒野生稻与栽培稻经体细胞杂交途径获得的新种质Y76中鉴定出的水稻抗白叶枯病新基因。将Y76与栽培稻＂大粒香＂回交构建F2群体，获得132个抗性稳定的株系。经致病菌株P6对该F2群体抗性鉴定，得到抗感株系数分别为32和100。随机选用覆盖水稻12条染色体的412对SSR引物对亲本和F2代群体进行多态性分析。筛选出8对多态性好的引物标记，连锁遗传分析发现位于xa32（t）同侧的RM8216和RM20A与其连锁，从而将xa32（t）定位在第12染色体长臂上，遗传距离为6.9cm和1.7cm。
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| 关 键 词： | 水稻白叶枯病 分子标记定位 xa32（t） 体细胞杂交后代 |
| 收稿时间： | 2008-04-14 |
| 修稿时间： | 2008-06-08 |
Identifying and Mapping New Gene xa32(t) for Resistance to Bacterial Blight(Xanthomonas oryzae pv. oryzae, Xoo) from Oryza meyeriana L. |
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| RUAN Hui-hui,YAN Cheng-qi,AN De-rong,LIU Ren-hu and CHEN Jian-ping. Identifying and Mapping New Gene xa32(t) for Resistance to Bacterial Blight(Xanthomonas oryzae pv. oryzae, Xoo) from Oryza meyeriana L.[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(6): 170-174 |
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| Authors: | RUAN Hui-hui YAN Cheng-qi AN De-rong LIU Ren-hu CHEN Jian-ping |
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| Affiliation: | College of Plant Protection, Northwest A&F University, Yangling Shaanxi 712100, China;Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Zhejiang Provincial Key Laboratory of Plant Virology, Key Laboratory of Animal and Plant Virology, Ministry of Agricultural, Hangzhou Zhejiang 310021, China;Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Zhejiang Provincial Key Laboratory of Plant Virology, Key Laboratory of Animal and Plant Virology, Ministry of Agricultural, Hangzhou Zhejiang 310021, China;College of Plant Protection, Northwest A&F University, Yangling Shaanxi 712100, China;Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Zhejiang Provincial Key Laboratory of Plant Virology, Key Laboratory of Animal and Plant Virology, Ministry of Agricultural, Hangzhou Zhejiang 310021, China;Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Zhejiang Provincial Key Laboratory of Plant Virology, Key Laboratory of Animal and Plant Virology, Ministry of Agricultural, Hangzhou Zhejiang 310021, China |
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| Abstract: | Abstract:A new bacterial blight resistance gene xa32 (t) was identified from the descendant of somatic hybridization between Oryza meyeriana L.to O.saliva L.ssp.japonica.A F2 population including 132 plants was generated from a cross between the descendant of somatic hybridization and O.saliva L.ssp.japonica.The bacterial blight strain P6 was used to test the resistance or susceptible of this F2 population and the result is there were 32 resistance and 100 susceptible plants.A stochastic 412 SSR primer pairs covering 12 rice chromosomes were used for polymorphism survey of the parents and the F2 population.There were 8 SSR markers found on rice chromosome 12.Linkage analysis showed that the two markers RM8216 and RM20A which on the long arm chromosome 12 link with xa32(t),located on the same side.And the genetic distance was 6.9 cm and 1.7 cm. |
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| Keywords: | Bacterial blight Gene mapping xa32(t) Descendant of somatic hybridization |
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