新型固定化乙酰胆碱酯酶传感器的制备及氨基甲酸酯农残检测

以双醛纤维素(dialdehyde cellulose,DAC)为固载酶基质材料,通过固定化酶化学键联技术制备固定化乙酰胆碱酯酶(DAC-AChE),采用自组装法制备多层壳聚糖/固定化乙酰胆碱酯酶/功能化碳纳米管生物传感器(Chi/DAC-AChE/F-CNTs/GCE),利用交流阻抗法(AC impedance,ACI),循环伏安法(cyclic voltammetry,CV)及差分脉冲伏安法(differential pulse voltammetry,DPV)考察新型传感器对农残的检测性能。结果表明:成功制备了新型固定化乙酰胆碱酯酶电化学传感器;新型传感器对氨基甲酸酯杀虫剂1-萘基-N-甲基氨基甲酸酯(carbary,CAB)具有良好的响应,检测方法响应快速,线性范围宽,灵敏度高,最低检出限可达3.5 μg·L-1,具有很好的重现性和稳定性,有关结果可为氨基甲酸酯类杀虫剂的检测提供一种新的分析方法。

A new biosensor from immobilized acetylcholinesterase to detect carbamate pesticides

 Dialdehyde cellulose（DAC）, an immobilized enzyme matrix material，was used to prepare an immobilized acetylcholinesterase according to immobilized enzyme chemical linking technology. The immobilized acetylcholinesterase was then used to prepare a new multilayer chitosan / immobilized acetylcholinesterase/functionalized carbon nanotube / glassy carbon electrode （Chi/DAC-AChE/F-CNTs/GCE） biosensor. AC impedance （ACI）, cyclic voltammetry（CV）, and differential pulse voltammetry（DPV） were utilized to characterize the detection performance of pesticides acting on the biosensor. Results showed that the new Chi/DAC-AChE/F-CNTs/GCE biosensor was successfully prepared and had a good response to carbaryl （CAB）. This new sensor provided a quick and easy detection method with a wide range and a high sensitivity having a minimum detection limit of 3.5 μg·L^－1 with good reproducibility and stability. These results could provide a new analytical method for the detection of carbamate pesticides. ［Ch, 6 fig. 4 tab. 25 ref.］