| 核桃早实性状相关联的RAPD标记 |
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| 引用本文： | 王国安,张虎平,虎海防,牛建新,马兵钢.核桃早实性状相关联的RAPD标记[J].果树学报,2004,21(5):485-487. |
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| 作者姓名： | 王国安 张虎平 虎海防 牛建新 马兵钢 |
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| 作者单位： | 1. 新疆林业科学院,新疆,乌鲁木齐,830000
2. 石河子大学农学院园艺系,新疆,石河子,832003 |
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| 基金项目： | 新疆自然科学基金资助项目(211003) |
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| 摘 要： | 运用RAPD技术进行核桃早实性状相关的分子标记研究,用180个随机引物,共扩增出1600条DNA带,选出5个多态性引物,进一步用这5个引物进行单株确认,发现只有引物OPG15在早实类群的8个个体中扩增出一条710bp的特异带,而晚实类群的8个个体中无此特异带。初步分析认为,OPG15710可能是与核桃早实性状相关的分子标记,该标记需进一步确定。
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| 关 键 词： | 核桃 早实性状 RAPD |
| 文章编号： | 1009-9980(2004)05-485-03 |
| 修稿时间： | 2004年1月12日 |
Identification of a RAPD Marker Related to Early-bearing Characteristic of Walnut Seedlings |
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| Wang Guoan,Zhang Huping ,Hu Haifang,Niu Jianxin ,and Ma Binggang.Identification of a RAPD Marker Related to Early-bearing Characteristic of Walnut Seedlings[J].Journal of Fruit Science,2004,21(5):485-487. |
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| Authors: | Wang Guoan Zhang Huping Hu Haifang Niu Jianxin and Ma Binggang |
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| Institution: | Wang Guoan1,Zhang Huping 2,Hu Haifang1,Niu Jianxin 2*,and Ma Binggang 2 |
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| Abstract: | Random Amplified Polymorphic DNA(RAPD) was applied as a tool for detecting molecular markers related to the characteristic of early-bearing walnut seedlings. 180 primers were screened, and a total of 1 600 DNA bands were amplified. 5 polymorphic primers were identified, and each form was verified by using the 5 polymorphic primers. Only primer OPG15 amplified a 710 bp DNA band on 8 forms of early-bearing walnut seedlings, but the DNA band was not amplified on 8 forms of late-bearing walnut seedlings. The results tentatively showed that OPG15710 was a molecular marker related to the precocious characteristic for early-bearing walnut seedlings. |
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| Keywords: | Walnut Early-bearing characteristic RAPD |
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