| 心肌型脂肪酸结合蛋白的纯化及亲和配体的研究 |
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| 引用本文： | 张淑华,范丽颖,何秀霞,于源华,王丽萍,侯巍. 心肌型脂肪酸结合蛋白的纯化及亲和配体的研究[J]. 吉林农业大学学报, 2006, 28(6): 628-630,638 |
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| 作者姓名： | 张淑华 范丽颖 何秀霞 于源华 王丽萍 侯巍 |
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| 作者单位： | 长春理工大学生命科学学院,长春,130022;吉林大学生命科学学院,长春,130021;中国人民解放军第461医院,长春,130021 |
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| 摘 要： | 利用盐析、G-75凝胶过滤层析和DEAE-葡聚糖纤维素离子交换层析技术,从牛心中分离出心肌型脂肪酸结合蛋白,经SDS-PAGE鉴定与免疫印迹(Western blot)分析,获得了纯品H-FABP。采用噬菌体随机展示七肽库,以H-FABP为靶标,对其亲和配体进行筛选,结果获得了与H-FABP有特异性亲和作用的配体序列,为其临床检测急性心肌梗塞新方法的建立奠定了基础。
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| 关 键 词： | 心肌型脂肪酸结合蛋白 层析 免疫印迹 亲和配体 |
| 文章编号： | 1000-5684（2006）06-0628-03 |
| 收稿时间： | 2006-03-12 |
| 修稿时间： | 2006-03-122006-09-28 |
Study on Purification and Affinity Ligand of Heart-Type Fatty Acid Binding Protein |
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| ZHANG Shu-hua,FAN Li-ying,HE Xiu-xia,YU Yuan-hua,WANG Li-ping,HOU Wei. Study on Purification and Affinity Ligand of Heart-Type Fatty Acid Binding Protein[J]. Journal of Jilin Agricultural University, 2006, 28(6): 628-630,638 |
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| Authors: | ZHANG Shu-hua FAN Li-ying HE Xiu-xia YU Yuan-hua WANG Li-ping HOU Wei |
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| Affiliation: | 1. College of Life Science, Changchun University of Science and Technology, Changchun 130022, China ; 2. College of Life Science, Jilin University, Changchun 130021, China ; 3. No.461 Hospital, the PLA , Changchun 130021, China |
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| Abstract: | In this study we obtained H-FABP from bovine heart muscle using DEAE-Sephacel ion-exchange chromatography and Sephacryl G-75 gel filtration chromatography.After SDS-PAGE and western blotting we got the pure product.The specific sequence of affinity ligand for H-FABP was obtained with the Ph.D.-7 Phage Display Peptide Library Kit subsequently.The study makes a new way to detect AMI in clinic. |
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| Keywords: | heart-type fatty acid binding protein chromatography western blot affinity ligand |
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