Detailed physicochemical characterization of the 2S storage protein from rape (Brassica napus L.) |
| |
Authors: | Schmidt Isabelle Renard Denis Rondeau David Richomme Pascal Popineau Yves Axelos Monique Augustin-Viguier |
| |
Affiliation: | Unité de Physico-Chimie des Macromolécules and Unité de Recherches sur les Protéines Végétales et leurs Interactions, INRA, Rue de la Géraudière, 44316 Nantes Cedex 03, France. |
| |
Abstract: | Chromatographic, chemical, and spectroscopic techniques were used to characterize the physicochemical properties of napin purified by preparative chromatography. The molar extinction coefficient was determined (epsilon = 0.56), and static and dynamic light scattering measurements enabled the average molecular weight (M(w) = 13919), the second virial coefficient (A(2) = 23.95 x 10(-)(5) mol cm(3) g(-)(2)), and the hydrodynamic radius (R(H) = 1.98 nm) to be determined. No conformational changes were observed by fluorescence and circular dichroism measurements in different buffers at pH 3, 4.6, 7, and 12, confirming the high pH stability of this protein. From MALDI-TOF analysis and after enzymatic digestion, it was found that this purified sample, extracted from the rapeseed variety Express, contained mainly isoform 2SS3_BRANA. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|