| Transformation of TrxS Gene into Barley by Particle Bombardment |
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| 引用本文： | WEI Li YIN Jun KONG Wei-wei REN Jiang-ping LI Lei LIU Lei. Transformation of TrxS Gene into Barley by Particle Bombardment[J]. 中国农业科学(英文版), 2005, 4(8): 574-578 |
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| 作者姓名： | WEI Li YIN Jun KONG Wei-wei REN Jiang-ping LI Lei LIU Lei |
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| 作者单位： | [1]Henan Agricultural University, National Engineering Research Center of Wheat, Zhengzhou 450002, P.R.China |
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| 摘 要： | The production of malting barley in China can＇t meet the demand of beer industries because of poor quality and it becomes a bottleneck problem in beer manufacture industry. In this paper, TrxS gene cloned from Phalaris coerulescens was transferred into barley cultivar Yupi 1 （YP1） via biolistic bombardment. 1206 immature embryos were bombarded and seven transgenic plants carrying TrxS gene were confirmed by PCR and PCR-Southern blotting analysis. TrxS gene was expressed in transgenic plants by RT-PCR analysis. The activity of Trxh and α-amylase of transgenic line were higher than that of non-transgenic line, which is helpful to improve malting quality of barley.
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| 关 键 词： | 大麦 TrxS基因 迁移行为 RT-PCR分析 |
Transformation of TrxS Gene into Barley by Particle Bombardment |
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| WEI Li,YIN Jun,KONG Wei-wei,REN Jiang-ping,LI Lei,LIU Lei. Transformation of TrxS Gene into Barley by Particle Bombardment[J]. 《Agricultural Sciences in China》, 2005, 4(8): 574-578 |
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| Authors: | WEI Li YIN Jun KONG Wei-wei REN Jiang-ping LI Lei LIU Lei |
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| Abstract: | The production of malting barley in China can't meet the demand of beer industries because of poor quality and it becomes a bottleneck problem in beer manufacture industry. In this paper, TrxS gene cloned from Phalaris coerulescens was transferred into barley cultivar Yupi 1 (YP1)via biolistic bombardment. 1206 immature embryos were bombarded and seven transgenic plants carrying TrxS gene were confirmed by PCR and PCR-Southern blotting analysis. TrxS gene was expressed in transgenic plants by RT-PCR analysis. The activity of Trxh and αt-amylase of transgenic line were higher than that of non-transgenic line, which is helpful to improve malting quality of barley. |
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| Keywords: | Barley TrxS gene Particle bombardment Transformation |
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