| HPLC法测定石斛夜光丸(大蜜丸)中盐酸小檗碱的含量 |
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| 引用本文： | 王德甫,许乾丽,左鼎,熊慧林.HPLC法测定石斛夜光丸(大蜜丸)中盐酸小檗碱的含量[J].安徽农业科学,2010,38(20):10637-10638,10673. |
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| 作者姓名： | 王德甫 许乾丽 左鼎 熊慧林 |
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| 作者单位： | 贵州省药品检验所,贵州贵阳,550004;贵州省药品检验所,贵州贵阳,550004;贵州省药品检验所,贵州贵阳,550004;贵州省药品检验所,贵州贵阳,550004 |
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| 摘 要： | 目的]建立用高效液相色谱测定石斛夜光丸中盐酸小檗碱含量的方法。方法]采用C18柱,流动相为乙腈-0.34%磷酸二氢钾-0.17%十二烷基磺酸钠（48∶26∶26）混合液,检测波长为345nm。结果]盐酸小檗碱在0.0428～1.1548μg/ml范围内线性关系良好,平均回收率为99.5%,RSD值为2.8%。结论]该分析方法简便,准确,可作为该制剂的定量分析方法。
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| 关 键 词： | 高效液相色谱法 石斛夜光丸 盐酸小檗碱 含量测定 |
HPLC Determination of Berberine Hydrochloride in Shihu Yeguang Pills |
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| Institution: | WANG De-fu et al(Guizhou Institute for Drug Control,Guiyang,Guizhou 550004) |
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| Abstract: | Objective] The aim was to develop an HPLC method to determine the contents of berberine hydrochloride in Shihu Yeguang pills.Method] The sample was analyzed by HPLC,using symmetry C18 column(250 mm×4.6 mm,5 μm),acetonitrile-0.34% potassium dihydrogen phosphate-0.17% sodium dodecylsulphate(48∶26∶26)as the mobile phase,and 345 nm as the detection wavelength.Result]The linear range of chlorogenic acid was 0.0427 7~1.154 80 μg/ml,the recovery rate was 99.5% and the RSD was 2.8%.Conclusion]The method is convenient,accurate and can be applied in quantitative determination of the medicine. |
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| Keywords: | HPLC Shihu Yeguang pills Berberine hydrochloride Content determination |
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