| 不同源性大肠杆菌多药耐药基因AcrA、AcrB部分基因的同源性分析 |
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| 引用本文： | 马红霞,邓旭明,欧阳红生,阎继业. 不同源性大肠杆菌多药耐药基因AcrA、AcrB部分基因的同源性分析[J]. 中国预防兽医学报, 2003, 25(2): 111-113 |
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| 作者姓名： | 马红霞 邓旭明 欧阳红生 阎继业 |
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| 作者单位： | 1. 吉林农业大学动物科技学院,吉林,长春,130118
2. 解放军军需大学动物科技系,吉林,长春,130062 |
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| 基金项目： | 吉林省科委资助项目 (吉科合字 990 2 2 2，2 0 0 10 5 38) |
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| 摘 要： | 通过聚合聚链式反应从不同源性大肠杆菌的染色体DNA上扩增出大肠杆菌多药耐药基因AcrA,AcrB的部分片段，将该片段分别连接到克隆载体pMD-18T的BamHI,XhoI酶切位点中，测定插入片段的核苷酸序列，并分别推导出其氨基酸序列。序列分析表明，不同源性大肠杆菌的AcrA的部分基因的核苷酸序列及所推导的氨基酸序列与GeneBank中该基因序列的同源性较高；不同源性大肠杆菌的AcrB的部分基因的核苷酸序列的同源性较低。所推导的氨基酸序列与GeneBank中该基因序列的同源性较高。
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| 关 键 词： | 大肠杆菌多药耐药基因 AcrA AcrB 测序 同源性分析 |
| 文章编号： | 1008-0589(2003)02-0111-03 |
| 修稿时间： | 2002-09-16 |
Homology Analysis of AcrA and AcrB Gene of Multiple Drug Resistance of Different Escherichia coli |
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| MA Hong_xia ,DENG Xu_ming ,OUYANG Hong_shen ,YAN Ji_ye. Homology Analysis of AcrA and AcrB Gene of Multiple Drug Resistance of Different Escherichia coli[J]. Chinese Journal of Preventive Veterinary Medicine, 2003, 25(2): 111-113 |
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| Authors: | MA Hong_xia DENG Xu_ming OUYANG Hong_shen YAN Ji_ye |
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| Affiliation: | MA Hong_xia 1,DENG Xu_ming 2,OUYANG Hong_shen 2,YAN Ji_ye 2 |
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| Abstract: | The part of AcrA and AcrB gene of multiple drug resistance of Escherichia coli were amplified by polymerase chain reaction(PCR) from DNA of Escherichia coli and fragments were ligated with the vector pMD_18T cut with BamH I and Xho I.Homology analysis of the part of AcrA and AcrB genes nucleotides sequence and deduced amino acids sequence revealed that the part of AcrA of different Escherichia coli had most similarity with that gene of GenBank,nucleotides sequence of the part of AcrB of different Escherichia coli had less similarity with that gene of GenBank,deduced amino acids sequence of the part of AcrB of different Escherichia coli had most similarity with that gene of GenBank. |
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| Keywords: | AcrA and AcrB gene of multiple drug resistance of Escherichia coli Sequencing Homology analysis |
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