| 小鼠肝再生过程中脂质代谢相关通路中基因的表达谱变化 |
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| 引用本文： | 袁运生,张夕原,严德珺,杨婷旭,郜尽,俞雁.小鼠肝再生过程中脂质代谢相关通路中基因的表达谱变化[J].农业科学与技术,2009,10(2):41-45. |
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| 作者姓名： | 袁运生 张夕原 严德珺 杨婷旭 郜尽 俞雁 |
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| 作者单位： | 袁运生,郜尽,俞雁,YUAN Yun-sheng,GAO Jin,YU Yan(上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海,200240;上海交通大学农业与生物学院动物科学系,上海,200240);张夕原,严德珺,杨婷旭,ZHANG Xi-yuan,YAN De-jun,YANG Ting-xu(上海交通大学农业与生物学院上海市兽医生物技术重点实验室,上海,200240;上海交通大学农业与生物学院食品科学与工程系,上海,200240)
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| 基金项目： | 国家科技部重大攻关课题 |
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| 摘 要： | 1材料与方法
1．1材料 试验动物：9周龄的雄性C57BIV6小鼠，自由采食和饮水，每天光照12h，黑暗12h。试剂：TRIzol试剂（Invitrogen）；RNeasy Total RNA Mini Kit（Qiagen）；基因芯片Genechip mouse genome 43020（Affymetrix）；17启动子引物序列（Affymetrix）。
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| 关 键 词： | 基因芯片 脂质代谢 再生过程 小鼠 表达谱 通路 TRIzol Total |
Expression Profile Changes of Genes Involved in Lipid Metabolism Pathway During Liver Regeneration in Mice |
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| YUAN Yun-sheng,ZHANG Xi-yuan,YAN De-jun,YANG Ting-xu,GAO Jin,YU Yan.Expression Profile Changes of Genes Involved in Lipid Metabolism Pathway During Liver Regeneration in Mice[J].Agricultural Science ＆ Technology,2009,10(2):41-45. |
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| Authors: | YUAN Yun-sheng ZHANG Xi-yuan YAN De-jun YANG Ting-xu GAO Jin YU Yan |
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| Institution: | 1. Shanghai Municipality Key Laboratory for Veterinary Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240; 2. Department of Animal science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240; 3. Department of Food Science and Engineering, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240) |
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| Abstract: | Objective] The aim of the research was to study the expression profile changes of genes involved in lipid metabolism pathway during liver regeneration in mice. Method] The CCl4 induced mouse model of liver regeneration was established and the total RNA was isolated from liver tissue of mouse. Then the changes of genes involved in lipid metabolism pathway during different stages of liver regeneration were detected through micro-array chip gene technique and their specific functions were also analyzed. Result] During the process of liver regeneration, the expression level of 98 genes involved in lipid metabolism pathway changed, which were divided into eight groups according to change trend. In the mass, the expression of genes was inhibited in the early stage and up-regulated in the late phase. And the gene expression associated with fatty acid synthesis pathway was mainly up-regulated while the catabolic pathway did not change significantly. Most of genes involved in bile acid synthesis pathway were suppressed before 4.5 d and up-regulated after 4.5 d or 7 d. Conclusion] During the process of liver regeneration, the genes associated with lipid metabolism are expressed in different trends, and this data should provide a specific range of genes for further studying the regulation effect of lipid metabolism related pathway on liver regeneration. |
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| Keywords: | Lipid metabolism Gene expression profiles Liver regeneration Micro-array chip |
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