HPLC法测定抗骨增生片中淫羊藿苷含量研究 |
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引用本文: | 张丁华,王德志,王艳丰,刘秀梅. HPLC法测定抗骨增生片中淫羊藿苷含量研究[J]. 中兽医医药杂志, 2010, 29(3): 29-31 |
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作者姓名: | 张丁华 王德志 王艳丰 刘秀梅 |
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作者单位: | 1. 河南农业职业学院动物科学系,河南中牟,451450 2. 商丘市第四人民医院 3. 北京大学医学部 |
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基金项目: | 河南省重大科技攻关项目,商丘市科技攻关项目 |
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摘 要: | 为建立抗骨增生片中淫羊藿苷含量的高效液相色谱(HPLC)测定方法,采用LC-10ATVP型高效液相色谱仪,流动相:乙腈-0.1%磷酸溶液(27∶73),流速:1.0 mL/min,检测波长:270 nm。结果显示,淫羊藿苷在0.104-0.520μg范围内呈良好线性关系(r=0.999 9);淫羊藿苷含量不少于0.20 mg/片;平均回收率为98.497%,RSD为1.217%。表明该方法简便,专属性强,重复性好,可用于该产品的质量控制。
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关 键 词: | 高效液相色谱法 抗骨增生片 淫羊藿苷 含量测定 |
Determinnation on the contents of icariin in Kanggu-zengsheng tablets by HPLC |
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Affiliation: | ZHANG Ding-hua,WANG Yan-feng(Department of Animal Sciences,Henan Vocational College of Agriculture,Zhongmu Henan 451450,China) |
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Abstract: | To determine the content of icariin in Kanggu-zengsheng tablets by HPLC,LC-10ATVP-ODS-C18(4.6 mm×250 mm,5 μm) was used,the mobile phase was consisted of acetonitrile-0.1% phosphoric acid(27∶73),the flow rate was 1.0 mL/min,the detecting wavelength was 270 nm.Results:The method was linear with the range of 0.104-0.520 μg for icariin(r=0.999 9);Icariin contents in Kangguzengsheng tablets was more than 0.20mg/tablet.The average recovery was 98.497% and RSD was 1.217%.The methods are simple with strong specificity and good reproducibility,and can be used as the quality control of this preparation. |
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Keywords: | HPLC Kangguzengsheng tablets icariin determination |
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