Identification of Specific RAPD Markers Linked to Anthracnose Resistant Gene in Native Wild Grapes of China |
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| Authors: | WANG Xi-ping WANG Yue-jin ZHOU Peng ZHENG Xue-qin |
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| Institution: | 1. College of Horticulture, Northwestern Science and Technology University of Agriculture and Forestry, Yangling , Shaanxi 712100
2. National Key Biotechnology Laboratory for Tropical Crops, SCATC, Haikou 571101 |
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| Abstract: | Randomly amplified polymorphic DNA (RAPD) was employed to detect molecular markers linked to anthracnose ( Spheceloma ampelinum de Bary) resistant gene in the native wild grapes ( Vitis L. ) of China. RAPD marker OPJ13-300 was linked to anthracnose resistant gene using 90-3 cross F1 V. quinquangularis Rehd (shang-24) × V. vinifera (Longyan). The marker was verified in 90-3 cross F1, Chinese wild grapes and V. riparia and European grape cuitivars. This work has provided a solid basis for molecular marker-assisted selection (MAS) to disease resistance and cloning of disease resistant genes. |
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| Keywords: | The wild grapes China Anthracnose resistance RAPD |
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