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大蒜总黄酮提取工艺研究
引用本文:张泽英.大蒜总黄酮提取工艺研究[J].安徽农业科学,2009,37(22):10673-10674.
作者姓名:张泽英
作者单位:武汉生物工程学院生物工程系,湖北武汉,430415
基金项目:武汉市市属高等学校科学(教学)研究项目 
摘    要:目的]研究有机溶剂浸提法提取大蒜黄酮类物质。方法]以乙醇-水为溶剂,以芦丁为标准品,采用分光光度法定量分析大蒜提取液中总黄酮含量。采用正交试验考察了乙醇浓度、料液比、提取温度、提取时间4个因素对大蒜黄酮提取率的影响。结果]最佳提取条件为:乙醇体积分数75%,液固比16:1,提取时间3h,提取温度70℃。在该优化条件下,大蒜黄酮含量为3.76%。结论]该方法操作方便,所需时间短,提取溶剂用量少,便于推广和普及,是提取大蒜总黄酮的有效方法。

关 键 词:大蒜  总黄酮  乙醇  提取工艺

Optimization of Extraction Technology of Garlic Flavones by Orthogonal Test
ZHANG Ze-ying.Optimization of Extraction Technology of Garlic Flavones by Orthogonal Test[J].Journal of Anhui Agricultural Sciences,2009,37(22):10673-10674.
Authors:ZHANG Ze-ying
Institution:ZHANG Ze-ying (Department of Bioteehnology, Wuhan Bioengineering Institute, Wuhan, Hubei 430415 )
Abstract:Objective] The process of extracting flavones from garlic by the method of organic solvent immersion extraction was studied.Method] The flavanoids were extracted by ethanol-water as the solvent from garlic and the content was determined by spectrophotometry.Orthogonal experiment was used to study the extraction technology of flavones in garlic.Effects of ethanol-water solvent,ratio of liquid to material,extraction time,extraction temperature on extraction rate of flavones were investigated.Result] The op...
Keywords:Garlic  Flavones  Ethanol  Extraction
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