A comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm |
| |
| Authors: | CE McGregor CA Lambert MM Greyling JH Louw L Warnich |
| |
| Institution: | (1) ARC Roodeplaat, Vegetable and Ornamental Plant Institute, Private Bag X293, Pretoria, 0001, Republic of South Africa;(2) Department of Genetics, University of Stellenbosch, Private Bag X1, Matieland, Stellenbosch, 7602, Republic of South Africa |
| |
| Abstract: | Several DNA marker systems and associated techniques are available today for fingerprinting plant germplasm but information
on their relative usefulness in particular crops is limited. The study investigated PCR based DNA fingerprinting in a set
of 39 potato cultivars using RAPDs (20 primers), ISSRs (6 primers), AFLPs (2 primers) and SSRs (5 primer pairs). Results show
that each of the four techniques can on their own, individually identify each cultivar, but that techniques differ in the
mean number of profiles generated per primer (or primer pair) per cultivar, referred to as Genotype Index (GI). The order
of merit based on this criterium and in this material was AFLPs (GI = 1.0), a multi-locus SSR (GI = 0.77),RAPDs (GI = 0.53),
ISSRs (GI = 0.47) and single locus SSRs (GI = 0.36). Problems in relating banding patterns to individual loci and alleles
for polyploid genomes, using these techniques as they are currently employed, are also discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
| |
| Keywords: | DNA fingerprinting genotype index molecular markers Solanum tuberosum |
| 本文献已被 SpringerLink 等数据库收录！ |
|
