野生大豆接种大豆疫霉根腐病后苯丙氨酸解氨酶(PAL)活性的变化

对大豆疫霉根腐病菌胁迫下抗感不同野生大豆品种根、茎、叶中苯丙氨酸解氨酶(PAL)活性的变化规律进行了研究.结果表明:在接种疫霉根腐病菌1号生理小种后,抗病野生大豆根和攀中的PAL活性在病程的大部分阶段比相应对照增加,并且变化的幅度较大,而感病品种相反.抗感野生大豆叶中PAL活性与对照相比变化幅度均较小.

Response of PAL Activity to Phytophthora sojae Inoculation in Glycine soja

Phytophthora root and stem rot caused by Phytophlhora sojae is a destructive disease in soybean production regions all around the world. Some protective enzymes begin to be active when soybean is infected by P. sojae. Of the enzymes, phenylalanine ammonia-lyase( PAL) is a key and rate-limiting enzyme in phenyl propane metabolize pathway which could produce kinds of antimicrobial products. There is abundant wild soybean in China,but there are few reports on the relationship about PAL and resistance of wild soybean to P. sojae. In this paper,4 resistant wild soybeans and 4 susceptible wild soybeans were inoculated with zoospores of P. sojae race 1 as treatment and those inoculated with water as control. The PAL activity in roots,stems,and leaves were measured after inoculation of 0,12,24,36,48,60,and 72 h in the treatment and control respectively. The results indicated that the PAL activity in roots and stems of resistant wild soybeans was higher than that of the control at most of the stages of pathogenetic process, but was lower than that of control for susceptible wild soybean. The change range of PAL activity in leaves of both susceptible and resistant wild soybeans were relatively low compared with that of control.