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| 烟草胞质雄性不育系与保持系线粒体DNA的RAPD分析 | |
| 引用本文： | 刘齐元,刘飞虎,黄海泉,何宽信,肖伏. 烟草胞质雄性不育系与保持系线粒体DNA的RAPD分析[J]. 江西农业大学学报, 2005, 27(6): 826-830 |
| 作者姓名： | 刘齐元 刘飞虎 黄海泉 何宽信 肖伏 |
| 作者单位： | 1. 江西农业大学,农学院,江西,南昌,330045 2. 云南大学,生命科学学院,云南,昆明,650091 3. 江西省烟叶科学研究所,江西,南昌,330045 |
| 基金项目： | 云南省农业生物技术重点实验室开放项目基金（2003E01）和江西省烟叶科学研究所资助项目 |
| 摘 要： | 用124个10碱基随机引物对烟草2对雄性不育系及其保持系线粒体DNA的RAPD分析表明，不育系与保持系间有112个引物未扩增出多态性片段，占引物总数的92．56％．这反映了不育系和保持系线粒体的同源性。同时，在不育系与保持系的线粒体基因组间也发现了明显的差异，这些差异片段与雄性不育的关系还需进一步研究。 |
| 关 键 词： | 烟草 胞质雄性不育系 线粒体DNA BAPD |
| 文章编号： | 1000-2286（2005）06-0826-05 |
| 收稿时间： | 2005-07-19 |
| 修稿时间： | 2005-07-19 |
RAPD Analysis of Mitochondrial DNAs of Cytoplasmic Male Sterile Line and Its Maintainer in Tobacco( Nicotiana tobacum) |
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| LIU Qi-yuan,LIU Fei-hu,HUANG Hai-quan,HE Kuan-xin,XIAO Fu. RAPD Analysis of Mitochondrial DNAs of Cytoplasmic Male Sterile Line and Its Maintainer in Tobacco( Nicotiana tobacum)[J]. Acta Agriculturae Universitis Jiangxiensis, 2005, 27(6): 826-830 | |
| Authors: | LIU Qi-yuan LIU Fei-hu HUANG Hai-quan HE Kuan-xin XIAO Fu |
| Affiliation: | 1. College of Agronomy , Jiangxi Agricultural University, Nanchang 330045, China; 2. Life Science College, Yunnan University, Kunming 650091; 3 Institute of Tobacco Science, Jiangxi Province, Nanchang 330045, China |
| Abstract: | The mitochondrial DNA of 2 CMS tobacco lines and their homolog maintainer lines were analysed by the method of random amplified polymorphic DNA using 124 random 10 - base primers. The result showed that 112 primers, 92.56% of total primers, did not amplified ploymorphic band between CMS and maintainer lines. This indicated the homolog of mitochondria between CMS and maintainer line. Meanwhile, some obvious differences in mitochondrial genome were detected between CMS and maintainer line. The relationship of these different bands in CMS to male sterility needs farther study. |
| Keywords: | tobacco cytoplasmic male sterility mitochondrial DNA RAPD |
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