湖南省食用百合生长期鳞茎腐烂病的病原鉴定

 龙牙百合为湖南省邵阳市的特色经济作物之一，随着栽培面积不断扩大，其病害发生日趋严重，尤其在田间生长后期鳞茎易出现褐变、腐烂、植株枯死等症状，严重影响百合生长，已成为遏制龙牙百合生产的重要因素之一。

Pathogen identification of edible lily(Lilium brownie var.viridulum) bulb rot during growth season in Hunan

Bulb rot disease is one of the most important restrictive factors on edible lily (Lilium brownie var. viridulum cv.Longya) production in Longhui County, Hunan. To investigate the causal agent, the rotted bulbs were collected from Longhui and kept in moist condition. Generally 3-septate falciform macroconidia and one-celled, ovoid or oval microconidia, typical of Fusarium oxysporum, were observed from the mold on the rot tissues under microscope. After a needle-pricking inoculation with isolate HNLH07 on healthy lily scales, rot symptom was reproduced. However, HNLH07 was failed to infect bulbs of gladiolus or tulip in a same inoculation test. PCR amplification was carried out by using universal rDNA-ITS primers. Sequence of PCR product showed 99% identity with those of F.oxysporum by BLAST analysis at NCBI. Phylogenetic analysis showed that HNLH07 was clustered to F.oxysporum with a high bootstrap value of 99%. It comes to a conclusion that the pathogen causing bulb rot of cv. Longya in Longhui County should be F.oxysporum f.sp.lilii.