麻栎橡子抗氧化及抗糖尿病活性的试验研究

通过在紫外285nm处测定抗氧化活性物质（蛋白质、芳族胺、酚类等化合物）的含量,在450nm处测定类胡萝卜素的含量,采用DPPH法测定不同溶剂橡子提取物的自由基消除能力,测定羟自由基消除活性及橡子提取物对α-葡萄糖苷酶的抑制作用,研究麻栎橡子抗氧化活性及抗糖尿病活性。结果表明：70%甲醇提取物中的抗氧化活性物质含量最高,而水提取物中最少;70%甲醇提取物和70%乙醇提取物的类胡萝卜素含量较高;70%甲醇提取物的DPPH自由基的消除能力最强;橡子的水提取物对羟自由基的清除作用最强;甲醇提取物的α-葡萄糖苷酶抑制率较高。说明麻栎橡子具有抗氧化活性和抗糖尿病作用。

Primary Study on Antioxidant Activities and Antidiabetic Properties of Quercus Acutissima Caruth Acorn

In the present study,the antioxidant activity and antidiabetic properties of Quercus acutissima caruth acorn were investigated.Protein,aromatic amine and phenol were detected under 285nm by UV-VIS SPECTROPHOTOMETER,and the content of carotenoid was detected under 450nm.The DPPH radical scavenging activity of types of acorn extracts was also tested.Futhermore,the hydroxyl radical scavenging activity and the anti-α-glucosidase activity of acorn extracts were tested.The results show that there were more protein,aromatic amine and phenol in 70% MeOH extracts,while there were the least in aqueous extracts of acorn.And there was more carotenoid in 70% MeOH extracts and 70% EtOH extracts.70% MeOH extracts had higher DPPH radical scavenging activity than other types of extracts.The aqueous extacts of acorn had the highest hydroxyl radical scavenging activity.The α-glucosidase inhibition of MeOH extracts was stronger than that of others.These results suggest that the acorn extracts may have antioxidant activities and antidiabetic activity.