基于野外大田试验的接菌紫穗槐生态修复效应研究

[目的]研究接菌紫穗槐对矿区退化植被的恢复生态效应,以期为丛枝菌根真菌应用于西部干旱半干旱煤矿区生态重建提供理论基础和野外试验基础数据。[方法]以紫穗槐为宿主植物,在野外大田条件下研究接种丛枝菌根真菌和紫穗槐的共生状况,以及对煤矿开采沉陷区植物根际土壤的改良作用。[结果]4a的连续监测结果表明,接菌促进了紫穗槐的生长,接菌紫穗槐成活率比对照高30%以上;接菌紫穗槐菌根侵染率和菌丝密度显著高于对照;接种菌根提高了紫穗槐根际土壤有效磷含量且降低了pH值,取得较好的生态修复效应。[结论]在野外大田条件下,接种菌根真菌能够促进植物—菌根共生关系的形成,改善植物—菌根共生体的营养环境。

Field Experiment of Ecological Restoration Application of Arbuscular Mycorrhizal Fungi Inoculation on Amorpha Fruticosa

[Objective] The ecological restoration effect of inoculation of Amorpha fruticosa in mining area of degraded vegetation was studied, to provide theoretical basis and field experimental data for the application of arbuscular mycorrhizal fungi in the ecological reconstruction of arid and semi-arid coal mines in Western China.[Methods] Take Amorpha fruticosa as the host plant, the symbiotic status of arbuscular mycorrhizal fungi and Amorpha fruticosa was observed in field conditions and the improvement of plant rhizosphere soil in coal mining subsidence area was studied.[Results] Through 4 years continual monitoring, the results showed that the inoculation promoted the growth of Amorpha fruticosa, inoculation survival rate was higher than 30%; the mycorrhizal infection rate and hyphal density of inoculated Amorpha fruticosa were significantly higher than those of the control; the rhizosphere soil available phosphorus of the inoculated Amorpha fruticosa increased and the pH value decreased. According to these, the ecological restoration effect was achieved.[Conclusion] Under field conditions, mycorrhizal fungi can promote the formation of plant-mycorrhizal symbiosis and improve the nutrient environment of plant-mycorrhizal symbiosis.