| 骨骼肌兴奋收缩偶联潜伏期肌浆网内Ca~(2 )变化的研究——细胞化学法及X-射线能量色散谱微区分析 |
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| 引用本文： | 杨勇骥,郑尊,夏金辉,邵晓良,姜永良.骨骼肌兴奋收缩偶联潜伏期肌浆网内Ca~(2 )变化的研究——细胞化学法及X-射线能量色散谱微区分析[J].浙江大学学报(农业与生命科学版),1998(Z1). |
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| 作者姓名： | 杨勇骥 郑尊 夏金辉 邵晓良 姜永良 |
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| 作者单位： | 第二军医大学基础部生物物理研究所 |
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| 摘 要： | 报道了采用红外探测－计算机控制的超低温快速冷冻固定技术、Ｘ－射线能量色散谱微区定量分析及Ｃａ２＋细胞化学技术对骨骼肌收缩潜伏期时肌浆网内的Ｃａ２＋进行了分析，从超微结构形态学的角度对骨骼肌兴奋－收缩偶联发生时，肌浆网内Ｃａ２＋的作用进行了研究，这对揭示骨骼肌兴奋－收缩偶联时，肌浆网内Ｃａ２＋的释放机理研究有重要意义。
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| 关 键 词： | 骨骼肌 X-射线能量色散谱微区定量分析 快速冷冻固定 |
Studying the change of Ca 2 during excitation contraction coupling in the sarcoplasmic reticulum of skeletal muscle by cytochemistry and EDX microanalysis Journal of Zhejiang Agricultural University |
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| Yang Yongji, Zheng Zun, Xia Jinghui, Shao Xiaoliang, Jiang Yongliang.Studying the change of Ca 2 during excitation contraction coupling in the sarcoplasmic reticulum of skeletal muscle by cytochemistry and EDX microanalysis Journal of Zhejiang Agricultural University[J].Journal of Zhejiang University(Agriculture & Life Sciences),1998(Z1). |
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| Authors: | Yang Yongji Zheng Zun Xia Jinghui Shao Xiaoliang Jiang Yongliang |
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| Abstract: | The Ca 2 changes of sarcoplasmic reticulum with the millisecond time course in skeletal muscle during excitation contraction coupling is reported in this paper A series of technology have been employed, which are two way infrared detectors with computer control, quick freezing fixation, cytochemistry and EDX microanalysis It has been obtained for the calcium changes in marcoplasmic reticulum at known millisecond time course following quick freezing after a single electrical stimulation |
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| Keywords: | skeletal muscle EDX microanalysis quick freezing fixation |
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