Androgenesis in Hexaploid Spring Wheat F2 Populations and Their Parents Using a Multiple-Step Regeneration System |
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Authors: | J Pauk O Manninen I Mattila Y Salo S Pulli |
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Institution: | J. Pauk;, Cereal Research Institute, H-6701 Szeged, FOB. 391 (Hungary) O. Manninen;, I. Mattila, Y. Salo and S. Pulli, Agricultural Research Centre, Institute of Plant Breeding, SF-31600 Jokioinen (Finland). |
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Abstract: | The main goals of the wheat androgenesis experiment were to study the main phenomena of in vitro androgenesis in anther culture of F2 populations (10) and their parents (6), to compare the usage of P-4 and C-17 media for the formation of embryo/callus and to demonstrate a new plant regeneration system. The P-4 induction medium was found to be significantly better than the C-17 in the number of responsive anthers (RA) and calli induced (CI) at the 1 % and 0.1 % level, respectively. Genotypic effect was evident in both RA and CI. The yields of F2 populations in RA and CI were significantly higher than those of their parents regarding both media. The data confirmed the existence of heterosis for RA and CI in F2 populations. The ratio of green/albino plant regeneration was more favourable in the C-17 derived embryo/calli than in the P-4 derived ones. The frequency of plantlet regeneration was enhanced in the group of unresponsive calli by the application of the multiple-step regeneration system. In this system the calli lacking well developed morphogenic structure were transferred to a new regeneration medium, containing a higher concentration of the same cytokinin, other cytokinin or basic medium, before the occurrence of irreversible changes in their physiology. |
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Keywords: | Triticum aestivum anther culture androgenesis regeneration haploids |
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