Phylogeny and nitrogen fixation potential of Bradyrhizobium species isolated from the legume cover crop Pueraria phaseoloides (Roxb.) Benth. in Eastern Cameroon |
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| Authors: | Papa Saliou Sarr Shigeru Araki Didier Aime Begoude Martin Yemefack Gabriel Ambroise Manga Takeo Yamakawa |
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| Institution: | 1. Center for African Area Studies, Graduate School of Asian and African Area Studies, Kyoto University, 46 Shimoadachi-cho, Sakyo-ku, 606-8501Yoshida, Kyoto, Japan;2. Regional Biocontrol and Applied Microbiology Laboratory, Institute of Agricultural Research for Development (IRAD), P.B. 2067, Yaoundé, Cameroon;3. IITA-Cameroon, 1st, Main Road IRAD, Nkolbisson, PO Box 2008 (Messa), Yaoundé, Cameroon;4. Institute of Agricultural Research for Development (IRAD), P.B. 2067, Yaoundé-Cameroon;5. Plant Nutrition Laboratory, Division of Molecular Biosciences, Department of Biosciences and Biotechnology, Faculty of Agriculture, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan |
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| Abstract: | Pueraria is an herbaceous, perennial legume crop originating in Asia. Pueraria phaseoloides (Roxb.) Benth. (tropical kudzu) is frequently introduced into production systems and is used as green manure, a cover crop and a forage plant, making it important economically. We used P. phaseoloides as a trap crop to study and characterize soil rhizobia in Eastern Cameroon. Bacteria were isolated from fresh nodules collected from field-grown P. phaseoloides roots. The 16S-23S rRNA internal transcribed spacer (ITS) sequences from 30 bacterial isolates were amplified by polymerase chain reaction (PCR) and the reaction products were sequenced. Phylogenetic analysis revealed that all isolates were ascribed to the genus Bradyrhizobium and were grouped into three clusters of Bradyrhizobium sp. strains, one cluster of B. yuanmingense strains, and one cluster of B. elkanii strains. Acetylene reduction assay (ARA) results indicated that the B. yuanmingense strains had significantly higher nitrogen fixation potential and that they could be used as inoculants to enhance nitrogen fixation in Pueraria grown in Eastern Cameroon. |
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| Keywords: | Pueraria phaseoloides phylogeny Bradyrhizobium N2-fixation Cameroon |
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