Investigation of acute toxicity of (2,4-dichlorophenoxy)acetic acid (2,4-D) herbicide on crayfish (Astacus leptodactylus Esch. 1823) |
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Authors: | A Ça?lan Karasu Benli Aylin Sepici-Dincel Duygu ?ahin |
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Institution: | a Turkish Republic, The Ministry of Agriculture and Rural Affairs, GDAPD (General Directorate of Agricultural Production and Development), Department of Aquaculture, Eski?ehir Yolu 9. Km Lodumlu, Ankara, Turkey b Department of Primary School Education, Gazi University, Teknikokullar, 06500 Ankara, Turkey c Department of Medical Biochemistry, Faculty of Medicine, Gazi University, 06510 Ankara, Turkey d Department of Biology Education, Gazi University, Teknikokullar, 06500 Ankara, Turkey |
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Abstract: | The acute 96 h LC50 of (2,4-dichlorophenoxy)acetic acid (2,4-D), a widely used agricultural herbicide, was determined on crayfish (Astacus leptodactylus Esch. 1823). Crayfish of 23.5 ± 1.49 g mean weight and 9.6 ± 0.21 cm mean length were selected for the bioassay experiments. The experiments were repeated three times, in 10 L tap water. The data obtained were statistically evaluated by the use of the E.P.A computer program based on Finney’s probit analysis method and the 96 h LC50 value for crayfish was calculated to be 32.6 mg/L in a static bioassay test system. 95% lower and upper confidence limits for the LC50 were 15.10-327.16. In conclusion, 2,4-D is highly toxic to crayfish, a non-target organism in the ecosystem. Water temperature was 23 ± 1 °C. Behavioral changes of crayfish were recorded for all herbicide concentrations. |
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Keywords: | (2 4-D) (2 4-Dichlorophenoxy)acetic acid Crayfish Astacus leptodactylus Acute toxicity LC50 |
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