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核果类果树总RNA的快速提取方法
引用本文:代永欣,杨相昆,王林. 核果类果树总RNA的快速提取方法[J]. 安徽农学通报, 2007, 13(7): 30-31. DOI: 10.3969/j.issn.1007-7731.2007.07.014
作者姓名:代永欣  杨相昆  王林
作者单位:新疆石河子大学农学院园艺系,新疆石河子,832003;新疆石河子大学农学院园艺系,新疆石河子,832003;新疆石河子大学农学院园艺系,新疆石河子,832003
摘    要:以核果类果树(桃、李、杏、扁桃)的叶片和皮层为材料,用两种经过改进的提取方法对其总RNA进行提取,结果均可得到纯度高、完整性好的总RNA,其中以一步法具有更加简单、快速、经济的特点.

关 键 词:核果类果树  总RNA  提取
文章编号:1007-7731(2007)07-30-02
收稿时间:2007-03-17
修稿时间:2007-03-17

Methods for Rapidly Extracting Total RNA from Stone Fruits
Dai Yongxin,Yang Xiangkun,Wang Lin. Methods for Rapidly Extracting Total RNA from Stone Fruits[J]. Auhui Agricultural Science Bulletin, 2007, 13(7): 30-31. DOI: 10.3969/j.issn.1007-7731.2007.07.014
Authors:Dai Yongxin  Yang Xiangkun  Wang Lin
Affiliation:Department of Horticulture, College of Agriculture, Shihezi University, Xinjiang 832003
Abstract:Leaves and cortex from stone fruits(peach,plum,apricot,almond) were used as material.RNA isolation methods suitable for stone fruits were screened out by modified SDS-method.RNAs of high purity and good integrity were obtained by the two modified methods,of which the one-step method was characterized with simplicity,swiftness and money-saving.
Keywords:Stone fruits  Total RNA  Isolation
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