| RP-HPLC法测定杜仲叶发酵液中绿原酸的含量 |
| |
| 引用本文： | 刘传安,邹盛勤.RP-HPLC法测定杜仲叶发酵液中绿原酸的含量[J].安徽农业科学,2005,33(10):1862-1863. |
| |
| 作者姓名： | 刘传安 邹盛勤 |
| |
| 作者单位： | 宜春学院理学院,江西,宜春,336000;宜春学院生物工程研究所,江西省天然药物活性成分研究重点实验室,江西,宜春,336000 |
| |
| 摘 要： | 建立反相高效液相色谱(HPLC)法测定杜仲叶发酵液中绿原酸的含量。色谱条柱SymmetrySheildRP18柱(3.9mm×300mm,5μm),以水-甲醇-乙酸(88∶12∶1.2,V/V)为流动相,流速1.0ml/min,检测波长326nm,柱温25℃。绿原酸对照品在2.24～11.2μg范围内线性关系良好(r=0.9993)。该方法简便、准确、线性范围宽,可用于含绿原酸的原料及产品的质量控制。
|
| 关 键 词： | 反相高效液相色谱法 杜仲叶 发酵液 绿原酸 |
| 文章编号： | 0517-6611（2005）10-1862-02 |
| 收稿时间： | 07 20 2005 12:00AM |
| 修稿时间： | 2005-07-20 |
Determination of the Chlorogenic Acid in Fermented Liquor of Eucommia ulmoides Oliv Leaf with RP-HPLC |
| |
| LIU Chuan-an et al.Determination of the Chlorogenic Acid in Fermented Liquor of Eucommia ulmoides Oliv Leaf with RP-HPLC[J].Journal of Anhui Agricultural Sciences,2005,33(10):1862-1863. |
| |
| Authors: | LIU Chuan-an |
| |
| Abstract: | A determination method of chlorogenic acid by high performance liquid chromtograph and photodiode array detector was established. The chromatographic column, Symmetry Sheild RP18 (3.9 mm×300 mm, 5 μm), water-methanol-acetic acid (88∶12∶1.2, V/V) mobile phase with 1.0 ml/min flow rate, the detected wavelength (326 nm), and the column temperature(25 ℃) were adopted. The calibration curve of chlorogenic acid was linear under the content of 2.24～11.2 μg, the correlation coefficient was 0.999 3. The method was simple, accurate and had wider linear range. The method can be adapted to control the quality of material and products that contained chlorogenic acid. |
| |
| Keywords: | RP-HPLC Eucommia ulmoides Oliv leaf Fermented liquor Chlorogenic acid |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
