羊草发育过程中同工酶的研究

采用聚丙烯酰胺凝胶电泳法 ,对羊草不同物候期不同器官的酯酶和过氧化物酶同工酶的多态性研究结果表明 :羊草酯酶同工酶活性在地上与地下营养器官间存在明显的互补性 ;过氧化物酶活性与有性生殖器官的发育有一定的一致性。总的来看 ,过氧化物酶同工酶与羊草的生长发育的关系较酯酶密切 ,可以作为生长发育特性评价较好生理指标 ;酯酶的稳定性较强 ,标志性酶带较恒定 ,可以作为遗传鉴定的指标。

Studies on the Isozymes in Leymus chinensis during Development

The multiplicity of isozymes of peroxidase and esterase by using polyacrylamide gel electrophoresis at different growing stages in different organs of L.chinensis was analyzed. The results showed that there were significant replenished each other in the enzyme activities of the esterase isozyme between belowground and aboveground vegetative organs of L.chinensis ;There are definite regularity between the enzyme activities of peroxidase and the development of reproductive organs?To sum up, it had a close relationship between the peroxidase isozyme and the development of L.chinensis,which could regarded as the physiological index of appraisement of development property;The stability of esterase and the permanent mark band could be regarded as the index of hereditary appraisal.