| 辣椒胞质雄性不育基因的分子标记 |
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| 引用本文： | 魏兵强,王兰兰,陈灵芝.辣椒胞质雄性不育基因的分子标记[J].西北农业学报,2010,19(10):166-168. |
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| 作者姓名： | 魏兵强 王兰兰 陈灵芝 |
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| 作者单位： | 甘肃省农业科学院,蔬菜研究所,兰州,730070 |
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| 基金项目： | 甘肃省农业生物技术研究与应用开发项目(GNSW2008-12); 甘肃省省自然科学研究基金计划项目(0803RJZA048) |
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| 摘 要： | 利用近等基因系原理和RAPD标记技术对辣椒胞质雄性不育基因组DNA及其保持系基因组DNA进行了比较分析,通过200条随机引物的RAPD扩增,获得了与不育基因连锁的RAPD标记BH19-S900。测序结果表明,不育标记BH19-S900序列全长864 bp。根据RAPD标记序列分别设计并合成双引物,将与不育基因连锁的标记BH19-S900转化为更为简单、稳定的SCAR标记SS730。
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| 关 键 词： | 辣椒 不育基因 RAPD SCAR |
RAPD and SCAR Markers Linked to Cytoplasmic Male Sterility Gene in Capsicum annuum L. |
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| WEI Bingqiang,WANG Lanlan and CHEN Lingzhi.RAPD and SCAR Markers Linked to Cytoplasmic Male Sterility Gene in Capsicum annuum L.[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(10):166-168. |
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| Authors: | WEI Bingqiang WANG Lanlan and CHEN Lingzhi |
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| Institution: | WEI Bingqiang,WANG Lanlan and CHEN Lingzhi (Vegetable Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China) |
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| Abstract: | In this paper,the cytoplasmic male sterility(CMS) genome and its maintainer genome were compared by RAPD and NILs(Near-isogenic lines) method with 200 random primers.One RAPD marker named BH19-S900 linked to CMS gene was found,the length was 864 bp.According to the sequences of BH19-S900,duplex primers were designed and synthesized.Finally,a SCAR marker SS730 had developed from RAPD markers BH19-S900 successfully. |
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| Keywords: | Pepper Sterility gene Maintainer gene RAPD SCAR |
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