| 快速检测大豆疫霉菌的酶联免疫吸附技术研究 |
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| 引用本文： | 窦坦德,李宝笃,沈崇尧.快速检测大豆疫霉菌的酶联免疫吸附技术研究[J].植物检疫,1997(3). |
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| 作者姓名： | 窦坦德 李宝笃 沈崇尧 |
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| 作者单位： | 济南植检所(窦坦德)，莱阳农学院(李宝笃)，北京农业大学(沈崇尧) |
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| 摘 要： | 利用已经制备的大豆疫霉菌的抗血清，对大豆疫霉菌的纯培养和大豆病组织进行检测。结果表明：间接法（Ｉ－ＥＬＩＳＡ）和夹心法（ＤＡＳ－ＥＬＩＳＡ）都能较好地检测纯培养的大豆疫霉菌，但在检测病组织时，夹心ＥＬＩＳＡ的特异性较强，能够可靠地检测到大豆病组织内的病原菌
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| 关 键 词： | 抗血清 大豆疫霉菌 间接ELISA 夹心ELISA 检测 |
Rapid detection of Phytophthora megasperma f.sp. glycinea by ELISA. |
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| Dou Tande.Rapid detection of Phytophthora megasperma f.sp. glycinea by ELISA.[J].Plant Quarantine,1997(3). |
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| Authors: | Dou Tande |
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| Abstract: | Antiserum was obtained with mycelial protein extract of one strain of Phytophthora megasperma f.sp. glycinea. 20 different strains of p. megasperma f.sp. glycinea in pure culture and the pathogen in naturlly infected or inoculated roots had been detected by ELISA. The results showed that direct double antibody sandwish ELISA(DAS ELISA) was more sensitive and specific than indirect ELISA for the detection of P. megasperma f.sp. glycinea in inoculated soybean, P.megasperma f.sp. glycinea was detected reliably by ELISA several days before oospores found or before symptoms developed. So DAS ELISA may be useful for quarantine or detection early of soybean roots infected with P.megasperma f.sp. glycinea. |
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| Keywords: | Antiserum Phytophthora megasperma f sp glycinea I ELISA DAS ELISA Detection |
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