响应面法优化超声辅助提取无花果叶功能性成分工艺

以无花果叶为试材,乙醇为提取试剂,采用超声辅助提取无花果叶中的功能性成分。以超声温度、乙醇浓度、料液比、超声时间为单因素,功能性成分(黄酮、补骨脂素、佛手柑内酯)得率为指标,在单因素试验的基础上,采用响应面法优化无花果叶功能性成分提取工艺。结果表明,最佳提取工艺条件为:超声温度72℃,乙醇浓度50%,料液比1∶45(g/mL),超声时间40 min,在该条件下,黄酮、补骨脂素、佛手柑内酯得率(37.93、11.56、2.02 mg/g)与预测值(38.11、11.56、2.05 mg/g)基本一致。

Optimization of Ultrasonic-Assisted Extraction Technique of Functional Components in Fig Leaves by Response Surface Methodology

With the grape seed polyphenols as research object, an immunosuppressive mice model was established through cyclophospamide (CPA) induction, and Illumina MiSeq high-throughput sequencing platform was used to explore the effect of grape seed polyphenols on the gut microbiota in immunosuppressive mice. The results showed that the grape seed polyphenols intervention could increase the immune organ index and the content of immunoglobulin(sIgA, IgM, IgG) in mice. Furthermore, the low does grape seed polyphenols could increase the diversity of gut microbiota, and promote the growth of Lactobacillus, Bacteroides, Parabacteroides distasonis, Bifidobacterium, Turicibacter, Alloprevotella tannerae, Allobaculum and Romboutsia, and inhibit the growth of Barnesiella, Escherichia/Shigella, Akkermansia. Meanwhile, the proliferation of probiotics such as Lactobacillus and Bifidobacterium was conducive to promoting the production of short-chain fatty acids, thus enhancing the immune ability of the body.