不同施肥条件下的小麦籽粒代谢组学研究

为探究不同肥料对小麦籽粒代谢物的影响,通过非靶向代谢组学结合气相色谱-质谱联用技术(GC-MS)与多元统计学的方法,对不同施肥条件下(常规施肥CK、缓控释肥一次性基施E、有机肥缓控释肥配施A、缓控释肥B、专家系统推荐施肥NE)的中信麦99籽粒代谢物进行分析。结果显示,从小麦籽粒中共检测到代谢物47种,主要为有机酸类、氨基酸类、糖类及其衍生物、酯类和其他有机物。与CK相比,B、E处理对促进小麦籽粒中有机酸类代谢物积累的效果较优,B、A处理对促进小麦籽粒中糖类代谢物积累的效果较优,B、NE处理对促进小麦籽粒中氨基酸类代谢物积累的效果较优;B、NE处理对促进小麦籽粒中总代谢物含量积累的效果最优,E处理效果次之,A处理效果最差。不同施肥处理对小麦籽粒代谢物中糖类和氨基酸类代谢物影响较大,通过富集20种差异代谢物,得到3条显著变化的代谢途径,分别为氨酰基-tRNA的生物合成,苯丙氨酸、酪氨酸和色氨酸的生物合成,异喹啉生物碱的生物合成。

Study on Metabonomics of Wheat Grain under Different Fertilization Conditions

In order to explore the effect of different fertilizers on wheat grain metabolites,in this experiment,non-targeted metabonomics combined with gas chromatography-mass spectrometry(GC-MS) and multivariate statistical methods were used to analyze the metabolites in grains of Zhongxinmai 99 under different fertilization conditions(conventional fertilization,CK; slow release fertilizer as base application,E; organic fertilizer combined with slow release fertilizer application,A; slow release fertilizer,B; andthe recommended fertilization by nutrient expert system,NE).The results showed that 47 kinds of metabolites were detected in wheat grains,mainly organic acids,amino acids,sugars and their derivatives,esters and other organic substances. Compared with CK,B and E had better accumulation effects on organic acid metabolites; B and A had better accumulation effects on carbohydrate metabolites; and B and NE hadbetter accumulation effects on amino acid metabolites. B and NE had the best effect on the accumulation of the overall metabolite content,followed by E,while A had the worst effect.Different fertilization conditions had a great impact on the carbohydrate and amino acid metabolites in wheat grain metabolites. By enriching 20 different metabolites,three significantly changed metabolic pathways are obtained: aminoacyl-tRNA biosynthesis,phenylalanine,tyrosine and tryptophan biosynthesis,and isoquinoline alkaloid biosynthesis.