氟苯尼考对铜绿微囊藻生长和生理特征的影响

试验以5个不同质量浓度氟苯尼考处理铜绿微囊藻,测定其生长及生理响应。结果表明,氟苯尼考质量浓度低于0.1 mg/L时促进铜绿微囊藻的叶绿素a合成,而对藻胆蛋白含量和多糖分泌无直接影响;质量浓度高于1.0 mg/L时则抑制其叶绿素a合成,而藻胆蛋白和多糖含量均随处理质量浓度升高而增加。不同质量浓度的氟苯尼考处理对铜绿微囊藻光合作用相关基因rbcL以及光系统Ⅱ反应中心蛋白编码基因psaB和psbD1均有一定程度的影响。rbcL和psaB基因的表达量在试验前3天均有下调,后期随质量浓度明显上调;psbD1基因在1.0 mg/L组的表达量明显高于其他组。表明水中氟苯尼考质量浓度在1.0 mg/L以上有促进铜绿微囊藻的生长趋势。

Effects of florfenicol on the growth and physiology of cyanobacteria (Microcystis aeruginosa)

Due to the improper disposal, antibiotic residues became one of the highly persistent pollutants and have obviously effective on the water ecosystem. In this paper, the growth characteristics, physiological condition and photosynthesis related genes of Microcystis aeruginosa were tested at five different treat concentrations of florfenicol. The results elaboreted that in lower concentrations group (lower than 0.1 mg/L), the contents of chlorophyll a had significantly increased, while no obvious significance on the phycobiliprotein content and the secretion of polysaccharide. In higher concentration group (higher than 1.0 mg/L), the synthesis of cyanobacteria chlorophyll a was inhibited, but the content of phycobiliprotein and polysaccharides secreted increased. The photosynthesis related genes rbcL, psaB and psbD1 of Microcystis aeruginosa were changed with florfenicol treatment group. The expression levels of rbcL and psaB genes were down-regulated in first three days, and up-regulation after 5th and 7th exposure. The expression level of the psbD1 gene in the 0.1 mg/L group was not significantly different with the control group at 3-5 days after treatment, while the 1.0 mg/L group was significantly up-regulated than that of other group. The results showed that 1.0 mg/L florfenicol in the water have a strong impact on growth of Microcystis aeruginosa