| 铁线莲愈伤组织及不定芽诱导试验 |
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| 引用本文： | 吴荣,樊国盛,王锦,林萍. 铁线莲愈伤组织及不定芽诱导试验[J]. 广东农业科学, 2014, 41(9): 48-50 |
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| 作者姓名： | 吴荣 樊国盛 王锦 林萍 |
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| 作者单位： | 西南林业大学园林学院，云南昆明650224 |
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| 基金项目： | 国家林业局“948”项目（2008-4-11）；云南省教育厅科研基金（09Y0308）；西南林业大学面上科研基金（2009M28）；云南省高校园林植物与观赏园艺重点实验室项目 |
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| 摘 要： | 以铁线莲Gipsy Queen的茎节和茎段为外植体，在MS基本培养基中添加0、1.0、2.0 mg/L 6-BA 和0、0.01、0.05 mg/L NAA进行初代诱导和继代增殖培养。结果表明院最适合诱导愈伤组织的培养基为MS+NAA 0.01 mg/L+6-BA2.0 mg/L，诱导率可达50.0%，诱导出的愈伤组织呈淡黄色疏松颗粒状；最适合诱导侧芽的培养基为MS+NAA 0.01mg/L+6-BA 1.0 mg/L，出芽率可达170%；最适增殖培养基为MS+NAA 0.01 mg/L+6-BA 2.0 mg/L，增殖倍数达3.39。
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| 关 键 词： | 诱导；愈伤组织；不定芽；铁线莲Gipsy Queen |
Study on induction of callus and adventitionsbuds in Clematis L. |
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| Abstract: | Callus and adventitions bud of Gipsy Queen were induced with columella and stem of Gipsy Queen asexplants and with the MS added different concentrations of 6-BA (0, 1.0, 2.0 mg/L) and NAA (0, 0.01, 0.05 mg/L) asnutrient medium. The results showed that the callus could be easily induced in MS+NAA 0.01 mg/L+6-BA 2.0 mg/L, theinduction rate was 50.0%, and the induced callus was loose and granular appeared yellowish. The best lateral bud inducedmedium was MS+NAA 0.01 mg/L+6-BA 1.0 mg/L, and the budding rate was 170%. The best multiplied medium was MS+NAA 0.01 mg/L+6-BA 2.0 mg/L, and the proliferation times was up to 3.39. |
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| Keywords: | induction callus adventitions bud Clematis Gipsy Queen |
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