| 胡椒木叶片离体培养与植株再生 |
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| 引用本文： | 李晓东,徐浩,张晓红,程智慧.胡椒木叶片离体培养与植株再生[J].西北农业学报,2009,18(2):213-216. |
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| 作者姓名： | 李晓东 徐浩 张晓红 程智慧 |
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| 作者单位： | 1. 深圳职业技术学院,城市园林与园艺系,广东深圳,518055
2. 西北农林科技大学,园艺学院,陕西杨凌,712100 |
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| 基金项目： | 深圳职业技术学院重点科研项目 |
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| 摘 要： | 探讨了胡椒木(Zanthoxylum piperitum)叶片的离体培养.选取胡椒木幼嫩叶片作为外植体,接种在不同激素组合的培养基上进行诱导培养,结果表明,最适的诱导培养基为MS+6-BA1.0 mg/L+NAA0.7 mg/L,分化率为91.7%,最适的增殖继代培养基为MS+6-BA1.0 mg/L+NAA0.7 mg/L,所产生的不定芽在1/2MS+ NAA0.4 mg/L的培养基上可全部生根,长成完整植株.首次证明以胡椒木的叶片为外植体可以进行快速离体培养.
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| 关 键 词： | 胡椒木 叶片 离体培养 植株再生 |
| 收稿时间： | 2008/9/15 0:00:00 |
| 修稿时间： | 2008/10/20 0:00:00 |
In Vitro Culture of Zanthoxylum piperitum Leaf Explants and Its Plantlet Regeneration |
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| LI Xiaodong,XU Hao,ZHANG Xiaohong and CHENG Zhihui.In Vitro Culture of Zanthoxylum piperitum Leaf Explants and Its Plantlet Regeneration[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(2):213-216. |
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| Authors: | LI Xiaodong XU Hao ZHANG Xiaohong and CHENG Zhihui |
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| Institution: | Department of Applied Biological Engineering, Shenzhen Polytechnic, Shenzhen Guangdong 518055, China;Department of Applied Biological Engineering, Shenzhen Polytechnic, Shenzhen Guangdong 518055, China;Department of Applied Biological Engineering, Shenzhen Polytechnic, Shenzhen Guangdong 518055, China;College of Horticulture, Northwest A&F University, Yangling Shaanxi 712100, China |
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| Abstract: | The aim was to study in vitro culture of leaves of Zanthoxylum piperitum.The young leaves from Zanthoxylum piperitum as explants were induced on media contain different combinations of hormones.The results showed that MS+6-BAl.0 mg/L+NAA 0.7 mg/L was the most appropriate media for differentiation of adventitious buds, shoot percentage was 91.7%,and the same medium was the most appropriate media for differentiation and multiplication of adventitious buds on.Adventitious purple shoots could be rooted and formed regenerated plants on 1/2MS medium supplemented with 0.4 mg/L NAA, and ratio of induced roots was 1000o.An efficient regeneration protocol was developed in the first time through shoot regeneration from leaf explants of Zanthoxylum piperitum. |
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| Keywords: | Zanthoxylum piperitum Leaves In vitro culture Plantlet regeneration |
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