屠宰场沙门氏菌耐药性分析和毒力基因检测

【目的】研究广东省茂名地区屠宰环节猪肉中携带的沙门氏菌的耐药性和毒力特征，为该地区食源性沙门氏菌的危害评估和防控措施制定提供依据。【方法】从广东省茂名地区屠宰场采集的猪肉、脾脏、肝脏样本中分离到19株沙门氏菌，采用K-B药敏纸片法检测其对β-内酰胺类、氟喹诺酮类、氨基糖苷类、四环素类、酰胺醇类和磺胺类抗菌药物的耐药性，用PCR方法检测β-内酰胺类耐药基因(bla_TEM、bla_OXA-1、bla_SHV)、氟喹诺酮类耐药基因(qnrA、qnrS、qnrB)、氨基糖苷类耐药基因(aadA1、aac(6′)-Ⅰb、rmtB)、四环素类耐药基因(tetA、tetB、tetC)、酰胺醇类耐药基因(Cat1、floR)、磺胺类耐药基因(SulⅠ、SulⅡ、SulⅢ)和10种毒力基因(mogA、sseL、mgtC、bcfA、araB、spvR、spvA、spvB、spvC、spvD)的携带情况。【结果】19株沙门氏菌耐药严重，对四环素、多西环素、氯霉素、氟苯尼考、磺胺异噁唑的耐药率均>50%,对四环素、多西环素和氯霉素的耐药率最高...

Drug Resistance Analysis and Virulence Gene Detection of Salmonella in Slaughterhouses

【Objective】 This study was aimed to investigate the drug resistance and virulence characteristics of Salmonella carried in slaughtered pork in Maoming,Guangdong province,and provide a basis for hazard assessment and formulation of prevention and control measures for foodborne Salmonella in this area.【Method】 Nineteen strains of Salmonella isolated from pork,spleen and liver samples collected from slaughterhouses in Maoming,Guangdong province were tested for resistance to β-lactams,fluoroquinolones,aminoglycosides,tetracyclines,aminoglycols and sulfonamides by the K-B drug-sensitive paper method,and the β-lactam resistance genes (bla_TEM,bla_OXA-1 and bla_SHV),fluoroquinolone resistance genes (qnrA,qnrS and qnrB),aminoglycoside resistance genes (aadA1,aac(6')-Ⅰb and rmtB),tetracycline resistance genes (tetA,tetB and tetC),aminol resistance genes (Cat1 and floR),sulfonamide resistance genes (SulⅠ,SulⅡ and SulⅢ) and 10 virulence genes (mogA,sseL,mgtC,bcfA,araB,spvR,spvA,spvB,spvC and spvD) were detected by PCR method.【Result】 19 strains of Salmonella were highly resistant to tetracycline,doxycycline,chloramphenicol,florfenicol and sulfaisoxazole,and the resistance rates were more than 50%,with the highest resistance rates of 89.5% (17/19) to tetracycline,doxycycline and chloramphenicol,and 89.5% to 3 or more antimicrobial drugs,with the highest resistance to all 11 antimicrobial drugs.The detection rate of bla_TEM,qnrA,aadA1, aac(6')-Ⅰb,Cat1,floR,SulⅠ and SulⅢ resistance genes was high (≥50.0%),the highest detection rate of bla_TEM resistance gene was 84.2%,73.7% of strains carried 5 or more resistance genes at the same time,and the highest strain carried 12 drug-resistant genes;mogA,mgtC,bcfA,araB virulence genes were all detected and the detection rate was above 70%,of which mogA virulence gene had a detection rate of up to 100%,and the remaining 6 virulence genes did not detect out.【Conclusion】 Salmonella from the pork of Maoming slaughterhouse in Guangdong province was seriously multidrug-resistance,carrying multiple drug-resistance genes with complex gene combinations and multiple virulence genes.