F3代波杂山羊VNN1基因多态性及其与产羔数的关联分析

【目的】 研究泛酰巯基乙胺酶-1(panthenoyl mercaptoethamine-1,VNN1)基因多态性与F3代波杂山羊产羔数的关系,从而从分子水平指导F3代波杂山羊的育种工作。【方法】 选取107只F3代波杂山羊,采集血样提取DNA,根据VNN1基因(登录号:NC_030816.1)外显子序列设计7对引物,进行PCR扩增及测序,运用DNAStar软件分析测序结果,对可能存在的单核苷酸多态性(SNP)位点外显子全群进行PCR扩增测序,并进行Hardy-Weinberg平衡状态及遗传多样性分析;应用SPSS 16.0统计学软件对VNN1基因不同基因型与F3代波杂山羊产羔数进行关联分析。【结果】 在VNN1基因在第5和7外显子中共发现7个SNPs位点:g.10956 A→G、g.11010 C→T、g.11103 C→T、g.11284 C→A、g.11313 T→C、g.18094 C→T和g.18158 G→C,且每个位点均存在3种不同基因型。χ²检验显示,g.10956 A→G、g.11010 C→T、g.11103 C→T、g.11313 T→C和g.18094 C→T位点均处于Hardy-Weinberg平衡状态,g.11284 C→A和g.18158 G→C位点均处于Hardy-Weinberg不平衡状态。多态信息含量均为中度多态((0.25<PIC<0.5)。关联分析发现,g.11010 C→T和g.11313 T→C位点与F3代波杂山羊产羔数显著相关,其中g.11010 C→T位点CC基因型产羔数显著低于CT和TT基因型,g.11313 T→C位点TT基因型产羔数显著低于TC和CC基因型(P<0.05);g.10956 A→G、g.11103 C→T和g.18158 G→C位点对F3代波杂山羊产羔数均无显著影响(P>0.05)。【结论】 VNN1基因在第5和7外显子中共发现7个SNPs位点,其中g.11010 C→T和g.11313 T→C位点对F3代波杂山羊产羔数有显著影响,VNN1基因可作为F3代波杂山羊的候选基因。

Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats

【Objective】 This experiment was conducted to investigate the relationship between panthenoyl mercaptoethamine-1 (VNN1) gene polymorphism and lambing number of F3 generation Boza goats,so as to provide molecular guidance for breeding of F3 generation Boza goats.【Method】 107 F3 generation Boza goats were selected for this experiment,blood samples were collected to extract DNA.According to the exon sequence of VNN1 gene(accession No.:NC_030816.1),7 pairs of primers were designed for PCR amplification and sequencing.DNAStar software was used to analyze the sequencing results,and PCR amplification and sequencing were performed on the entire group of possible single nucleotide polymorphism (SNP) sites.Hardy-weinberg equilibrium and genetic diversity analysis were carried out,and the correlation analysis between the different genotypes of VNN1 gene and the lamb number in F3 generation Boza goats was conducted by SPSS 16.0 statistical software.【Result】 There were 7 SNPs in exons 5 and 7 of VNN1 gene (g.10956 A→G,g.11010 C→T,g.11103 C→T,g.11284 C→A,g.11313 T→C,g.18094 C→T and g.18158 G→C),and 3 different genotypes existed in each site.χ² test showed that g.10956 A→G,g.11010 C→T,g.11103 C→T,g.11313 T→C and g.18094 C→T were in Hardy-Weinberg equilibrium.g.11284 C→A and g.18158 G→C were hardy-Weinberg disequilibrium.The polymorphic information content was moderate polymorphis (0.25<PIC<0.5).Correlation analysis showed that g.11010 C→T and g.11313 T→C were significantly correlation with the lamb number in F3 generation Boza goats,CC genotype of g.11010 C→T was lower than CT and TT genotypes;TT genotype of g.11313 T→C was significantly lower than TC and CC genotypes (P<0.05).There was no significant correlation between g.10956 A→G,g.11103 C→T and g.18158 G→C and the lamb number in F3 generation Boza goats (P>0.05).【Conclusion】 There were 7 SNPs in exons 5 and 7 of VNN1 gene,g.11010 C→T and g.11313 T→C of VNN1 gene were significantly correlated with the number of lambs in F3 generation Boza goats,and VNN1 gene could be used as a candidate gene.