复合添加剂对解冻后奶牛性控冻精质量及受胎率的影响

【目的】试验旨在研究以褪黑素和地诺前列腺素组成的复合添加剂对奶牛性控冻精解冻后精子质量及奶牛人工授精后受胎率的影响。【方法】30份奶牛性控冻精样均分为2组：试验组和对照组，试验组添加由24 ng/mL褪黑素、1.5 mg/mL地诺前列腺素及精液稀释液组成的250μL复合添加剂，对照组添加250μL精液稀释液，试验组和对照组均与解冻后的奶牛性控冻精按照1∶1(V/V)进行混合，室温孵育0、2及4 h,通过免疫荧光染色分析精子活率、顶体完整率、高能线粒体活性精子比率；对216头青年奶牛和82头头胎奶牛输精后通过28 d早孕检测来确定受胎率情况。【结果】与对照组相比，经复合添加剂处理0 h的性控冻精活率、顶体完整率及高能线粒体活性精子比率均无显著变化(P>0.05);经复合添加剂处理2 h的性控冻精活率、顶体完整率、高能线粒体活性精子比率均显著升高(P<0.05);经复合添加剂处理4 h的性控冻精活率和顶体完整率均无显著变化(P>0.05),高能线粒体活性精子比率显著升高(P<0.05)。在输精试验中，试验组青年奶牛和头胎奶牛中的受胎率分别为65.22%和48.21%...

Effects of Compound Additives on the Quality and Conception Rate of Dairy Cows Thawed Sex Controlled Frozen Semen

【Objective】 This study was aimed to investigate the effect of compound additive composed of melatonin and dinoprostatin on sex controlled frozen semen quality and conception rate after artificial insemination.【Method】 Thirty sex controlled frozen sperm of dairy cows was divided into experimental and control groups,250 μL compound semen additive composed of 24 ng/mL melatonin,1.5 mg/mL dinoprostatin and semen dilution was added to experimental group,and 250 μL semen dilution was added to control group.The experimental and control groups were mixed with thawed sex controlled frozen semen in dairy cows according to the volume of 1∶1, and incubated at room temperature for 0, 2 and 4 h. The viability rate, acrosome integrity rate and high-energy mitochondrial ratio were analyzed by immunofluorometric assay, and the conception rate of 216 heifers and 82 first lactating dairy cows were determined by 28 day early pregnancy test after artificial insemination. 【Result】 Compared with control group,there was no significant difference in the viability rate,acrosome integrity rate and high-energy mitochondria ratio after treatment with compound additives for 0 h (P＞0.05);The viability rate, acrosome integrity rate and high-energy mitochondria ratio were significantly increased after treatment with compound additives for 2 h (P＜0.05);There was no significant difference in the viability rate and acrosome integrity rate after treatment with compound additives for 4 h (P＞0.05),but the high-energy mitochondria ratio was significantly increased (P＜0.05).In the insemination test,the conception rates of heifers and first lactating dairy cows in experimental group were 65.22% and 48.21%,which were significantly higher than control group (P＜0.05).【Conclusion】 The compound additive composed of melatonin and dinoprostatin could promote the thawed semen quality of dairy cows and the conception rate of heifers and first lactating dairy cows after insemination.