大白猪ENO3基因多态性及其与生长性状的关联分析

目的] 挖掘大白猪β-烯醇化酶（β-enolase，ENO3）基因单核苷酸多态性（SNP）位点，分析SNP间的连锁不平衡程度及其与生长性状的相关性。方法] 选取大白母猪316头，采用混合DNA样本PCR扩增产物测序，确定ENO3基因SNP位点。采用GenoPlexs分型技术对每个个体的目标SNP位点进行基因分型，并与大白猪体重达100 kg时的日增重、日龄、背膘厚、眼肌面积和眼肌厚进行连锁不平衡和关联分析，探究ENO3基因SNP与大白猪生长性状的相关性。结果] 共鉴定出9个SNPs位点，均为已知SNPs，其中rs196953768与rs324943047、rs345530479、rs329283992完全连锁（D'=1，r²=1），与rs342598032呈强连锁（D'=1，r²=0.99）；rs327882211与rs341541240、rs325279236呈强连锁（D'=1，r²>0.7）。ENO3基因rs196953768及完全连锁位点与大白猪体重达100 kg时的日龄和日增重均呈显著相关（P<0.05）；rs327882211和rs341541240位点与体重达100 kg时的眼肌面积和眼肌厚均呈显著相关（P<0.05）；rs325279236与体重达100 kg时的眼肌面积呈显著相关（P<0.05）；rs786427749和rs342598032位点与上述各性状的相关性均不显著（P>0.05）。结论] 本研究共鉴定出9个SNPs，4个完全连锁的SNPs；筛选到7个与生产性状显著相关的SNPs，为大白猪的分子标记辅助选育提供了参考数据。

Polymorphism of ENO3 Gene and Its Correlation with Growth Traits in Yorkshire Pigs

Objective] The aim of this study was to explore the single nucleotide polymorphism (SNP) of enolase 3 (ENO3) gene, and analyze the linkage imbalance between SNPs and its correlation with growth traits in Yorkshire pigs. Method] A total of 316 sows were selected, the SNP of ENO3 gene were identified by PCR amplification product sequencing of mixed DNA samples. GenoPlexs typing technique was used to genotype the target SNP of each individual, the linkage disequilibrium and correlation analysis were conducted with daily gain, days, backfat thickness, loin-eye area and loin-eye thickness at 100 kg body weight, and explored the correlation between ENO3 gene SNP and growth traits of Yorkshire pigs. Result] The results showed that 9 SNPs were identified, among which rs196953768 was completely linked to rs324943047, rs345530479 and rs329283992 (D'=1, r²=1), and strongly linked to rs342598032 (D'=1, r²=0.99), rs327882211 was strongly linked with rs341541240 and rs325279236 (D'=1, r²>0.7). ENO3 gene rs196953768 and its complete linkage locus were significantly correlated with days and daily gain at 100 kg body weight (P<0.05), rs327882211 and rs341541240 were significantly correlated with loin-eye area and loin-eye thickness at 100 kg body weight (P<0.05);rs325279236 was significantly correlated with loin-eye area at 100 kg body weight (P<0.05);rs786427749 and rs342598032 had no significant correlation with the above growth traits (P>0.05). Conclusion] In this study, a total of 9 SNPs were excavated, 4 fully linked SNPs were identified on ENO3 gene, 7 SNPs significantly correlated with growth traits were screened, it provided reference data for molecular marker assisted breeding of Yorkshire pigs.