| RNA干扰在植物抗根结线虫病基因工程应用中的研究进展 |
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| 引用本文： | 牛俊海,郭仰东,简恒.RNA干扰在植物抗根结线虫病基因工程应用中的研究进展[J].广西农业生物科学,2009(1). |
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| 作者姓名： | 牛俊海 郭仰东 简恒 |
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| 作者单位： | 中国农业大学农学与生物技术学院蔬菜系;中国农业大学农学与生物技术学院植病系线虫学实验室; |
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| 基金项目： | 公益性行业专项(NYHYZX07-050);;农业部948项目(2006-G54);;国家十一五支撑项目(2006BAD08A08)资助 |
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| 摘 要： | 植物根结线虫病对农业生产的危害连年加重,以轮作和化学农药为主的传统防治难以满足现代农业生产的需要。以常规的抗性育种和表达外源蛋白为主的抗线虫转基因育种主要受限于抗性基因的匮乏。而近来RNA干扰技术的应用为抗线虫基因工程带来新的突破,通过构建RNA干扰载体,在转基因植物中表达寄生线虫重要基因的dsRNA或siRNA,并经口针取食被导入线虫体内,并引发线虫的系统性RNA干扰反应,导致其出现寄生、发育、代谢、运动等障碍甚至致死,从而使转基因植物实现对寄生线虫的抗性。本文综述了RNAi介导的抗根结线虫基因工程方面的研究进展,分析探讨了这种新的策略的特点并展望了它的应用前景。
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| 关 键 词： | RNA干扰 双链RNA 根结线虫 基因工程 |
Progress in the Study of RNAi Mediated Plant Root-knot Nematodes Resistant Genetic Engineering |
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| Niu Junhai,Guo Yangdong,Jian Heng.Progress in the Study of RNAi Mediated Plant Root-knot Nematodes Resistant Genetic Engineering[J].Journal of Guangxi Agricultural and Biological Science,2009(1). |
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| Authors: | Niu Junhai Guo Yangdong Jian Heng |
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| Institution: | Niu Junhai 1 Guo Yangdong 1 Jian Heng 2 1 Department of Vegetable Science,College of Agriculture , Biotechnology,China Agricultural University,Beijing,100094,2 Laboratory of Nematology,Department of Plant Pathology |
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| Abstract: | Agricultural losses caused by plant root-knot nematodes is increasing continuely.Traditional control strategies such as crop rotation and nematicidal cannot meet the needs of modern agriculture development.Resistance breeding and genetic engineering mainly limited by the lack of resistance gene.Recently an emerging breakthrough in transgenic resistance breeding is the use of RNA interference technology,transgenic plants expressing dsRNA hairpin structures targeting root-knot nematode essential genes.dsRNA o... |
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| Keywords: | RNA interference dsRNA Root-knot nematode Genetic engineering |
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