| 李种质资源ISSR反应体系的建立 |
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| 引用本文： | 乔玉山,章镇,房经贵,沈志军,赵密珍. 李种质资源ISSR反应体系的建立[J]. 果树学报, 2003, 20(4): 270-274 |
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| 作者姓名： | 乔玉山 章镇 房经贵 沈志军 赵密珍 |
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| 作者单位： | 1. 南京农业大学园艺学院,江苏,南京,210095
2. 江苏省农业科学院园艺研究所,江苏,南京,210014 |
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| 摘 要： | 以5′-(AC)_9 A-3′为引物对牛心李(Prunus salicina cv.Niuxinli)ISSR(inter-simple sequence repeats)反应体系的优化研究表明,25μL ISSR反应体系中,Taq酶、Mg~(2+)、引物、模板DNA和dNTPs5种主要成分的最适浓度分别是:0.3U、1.5mmol/L、0.2μmol/L、20ng和0.16mmol/L。利用该优化体系,以5′-(AC)_9 T-3′为引物,构建了中国李18个品种的ISSR指纹图谱,该引物可将这些品种完全区别开来;以5′-(AC)_9 C-3′为引物,构建了李属6类种质资源的ISSR指纹图谱,该引物区分率为100％。
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| 关 键 词： | 李 品种 种质资源 ISSR |
| 文章编号： | 1009-9980(2003)04-270-05 |
| 修稿时间： | 2003-01-06 |
Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm |
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| QiaoYushan,Zhang Zhen,Fang Jinggui,ShenZhijun,and Zhao Mizhen. Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm[J]. Journal of Fruit Science, 2003, 20(4): 270-274 |
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| Authors: | QiaoYushan Zhang Zhen Fang Jinggui ShenZhijun and Zhao Mizhen |
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| Abstract: | The optimal reaction of ISSR (Inter-simple Sequence Repeats) in Prunus salicina cv. Niuxinli was studied with primer 5'-(AC)9A-3'. The results showed that the optimum concentration of five important components i.e. Taq DNA poly-merase, Mg2+, primer, template DNA and dNTPs in 25 μX reaction system of ISSR were 0.3U, 1.5 mmol/L, 0.2 μmol/L, 20 ng and 0.16 mmol/L, respectively. The ISSR fingerprinting of 18 Japanese plum cultivars and 12 plum species or variety were established by using optimum system with primer 5'-(AC)9T-3'and 5'-(AC)9C-3', respectively. The identification rate of plum cultivars and species or variety was 100%. |
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| Keywords: | Plum Cultivar Germplasm ISSR |
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