‘秦美’猕猴桃贮藏期病原真菌的鉴定

以‘秦美’猕猴桃为试材,分离纯化在贮藏过程中引起腐烂的主要病原真菌。采用形态学观察及核糖体rDNA-ITS(Internal transcribed spacer)区序列分析法进行鉴定。结果表明:从腐烂猕猴桃中共分离出5株致病菌,对ITS区序列测序结果经GenBank数据库BLAST比对,A的ITS序列与Trichothecium roseum(EU552162.1)同一分支,支持率达100%;B的ITS序列与Fusarium tricinctum(AB587078.1)同一分支,支持率达99%;C的ITS序列与Penicillium expansum(AF330635.1)同一分支,支持率达99%;D的ITS序列与Colletotrichum boninense(KF819619.1)同一分支,支持率达99%;E的ITS序列与Botrytis elliptica(KJ638600.1)同一分支,支持率达100%。由形态学及ITS区序列分析,构建系统发育树,最终鉴定A为粉红聚端孢(Trichothecium roseum),B为镰刀菌属(Fusarium tricinctum),C为扩展青霉(Penicillium expansum),D为炭疽病菌(Colletotrichum boninense),E为灰葡萄孢(Botrytis cinerea)。5种病原真菌中灰葡萄孢为主要致病菌。

Identification of Pathogenic Fungi from Qinmei Kiwifruit at Stage of Post-harvest Storage

With Qinmei kiwifruit as test material, the main pathogenic fungal were separated and sublimated from rot kiwifruit during it was stored. It was identified by sequence analysis of morphological and ribosomal rDNA- Internal transcribed spacer (ITS).Five pathogens was isolated from the rotten kiwifruit, then the results of the sequencing of ITS area were tested by using blast in GenBank database. The analysisof the sequence of A gene revealed 100% identity with Trichothecium roseum (EU552162.1);B gene revealed 99% identity with Fusarium tricinctum (AB587078.1); C gene revealed 99% identity with Penicillium expansum (AF330635.1);D gene revealed 99% identity with Colletotrichum boninense (KF819619.1) ; E gene revealed 100% identity with Botrytis elliptica (KJ638600.1). By sequence analysis of morphological and TTS,five pathogenic bacteria were designated as Trichothecium roseum,Fusarium tricinctum,Penicillium expansum,Colletotrichum boninense and Botrytis elliptica.Botrytis elliptica is the main pathogenic bacterias.