<Emphasis Type="Italic">Agrobacterium tumefaciens</Emphasis>-mediated transformation as a tool for random mutagenesis of <Emphasis Type="Italic">Colletotrichum trifolii</Emphasis> |
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Authors: | Hiroyuki?Takahara Gento?Tsuji Yasuyuki?Kubo Mikihiro?Yamamoto Kazuhiro?Toyoda Yoshishige?Inagaki Yuki?Ichinose Email author" target="_blank">Tomonori?ShiraishiEmail author |
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Institution: | (1) Laboratory of Plant Pathology and Genetic Engineering, Faculty of Agriculture, Okayama University, Okayama, 700-8530, Japan;(2) Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Prefectural University, Kyoto, Japan |
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Abstract: | We transformed Colletotrichum trifolii, the causal agent of alfalfa anthracnose, using Agrobacterium tumefaciens as a new tool for random insertional mutagenesis. Fungal spores of C. trifolii were transformed with T-DNA including the hygromycin phosphotransferase gene (hph). Southern analysis showed that every randomly selected transformant had a unique hybridization pattern of T-DNA, suggesting that the T-DNA was randomly integrated into the fungal genome. More significantly, about 75% of transformants had a single copy of the T-DNA. The results demonstrate that insertional mutagenesis via A. tumefaciens is a useful tool for studying the function of C. trifolii genes. |
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Keywords: | Colletotrichum trifolii Anthracnose Agrobacterium tumefaciens Random insertional mutagenesis Transformation T-DNA |
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