Analysis of antibody responses by commercial western blot assay in horses with alveolar echinococcosis |
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Authors: | Ueno Masahiro Kuroda Nobuhiko Yahagi Kazue Ohtaki Toshihiko Kawanaka Masanori |
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Institution: | Yamagata Prefectural Nairiku Meat Inspection Center, Yamagata 990-0892, Japan. |
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Abstract: | Commercial western blot (WB) assay was used to detect serum antibodies specific to Echinococcus multilocularis in 23 horses in which infection was confirmed by postmortem inspection at a slaughterhouse. Livers contained from 1 to >20 nodular lesions; foci diameter ranged from 1 to 25 mm. Antibody tests of serum from all 23 animals were negative for antigen bands at 7, 16, 18, and 26-28 kDa, which show specificity in the serum of human patients. However, sera from two infected horses with the largest nodules (diameter, 25 mm) showed positive response to one of the 22-kDa and 30-kDa antigen bands. It may be possible to diagnose E. multilocularis infection in horses based on the detection of these bands on commercial WB assay. |
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