3种虾类超氧化物歧化酶同工酶比较

目的]比较日本对虾、克氏原螯虾、凡纳滨对虾不同组织的超氧化物歧化酶(SOD)同工酶差异。方法]将供试材料进行预处理后,测定各材料SOD活性,并采用聚丙烯酰胶凝胶电泳分析同工酶差异。结果]SOD活性存在组织差异和种属差异性;电泳谱带在3种虾类中也存在着明显的差异,其中日本对虾和凡纳滨对虾的SOD的电泳谱带和迁移率较为相似,与克氏原螯虾存在明显不同。结论]SOD同工酶活性比较可揭示虾类的亲缘关系与起源。

Comparison of Superoxide Dismutase Isoenzymes in Three Shrimps

Objective] The aim of the study was to compare the activities of superoxide dismutase(SOD) isoenzymes in three species of shrimps Penaeus japonicus,Procambarus clarkia and Litopenaeus vannamei.Method] The experimental materials were used to measure SOD activities after pretreatment,meanwhile differences in SOD isoenzymes from different materials were assayed by polyacrylamide gel electrophoresis(PAGE).Result] There are species specific differences in shrimps.With an similar electrophoresis pattern and migration rate,Penaeus japonicus and Litopenaeus vannamei showed remarkable differences with that of Procambarus clarkia.Conclusion]The result showed the differences of cognation and origin of three shrimps.