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| 肉鸡肠道CDX2 mRNA表达的肠段差异性与发育性变化 | |
| 引用本文： | 詹勋,冯幼,王修启,束刚,江青艳,冯定远,舒绪刚.肉鸡肠道CDX2 mRNA表达的肠段差异性与发育性变化[J].华南农业大学学报,2009,30(2). |
| 作者姓名： | 詹勋 冯幼 王修启 束刚 江青艳 冯定远 舒绪刚 |
| 作者单位： | 1. 华南农业大学增城正大肉鸡中心,广东广州,510642 2. 华南农业大学动物科学学院,广东广州,510642 3. 广州天科科技有限公司,广东广州,510627 |
| 基金项目： | 国家自然科学基金，国家重点基础研究发展规划(973计划) |
| 摘 要： | 选用遗传背景相同的1日龄父母代雄性Arbor Acre(AA)肉雏鸡120羽,随机分为4个重复,采用相对定量RT-PCR方法,以从肉鸡30 d肠道样品为模板,研究肉鸡肠道尾形相关同源盒基因(cadua-related type homeoboxgene)家族中CDX2 mRNA表达的组织特异性;以从肉鸡十二指肠和空肠样品为模板,研究肉鸡肠道CDX2 mBNA表达的发育性变化.结果显示:AA肉鸡十二指肠CDX2 mRNA的表达丰度高于空肠(P=0.09)、回肠(P=0.06)和结直肠(P=0.05);从肉鸡CDX2 mRNA在十二指肠及空肠中的表达具有相同的发育模式,2～30 d下降,44 d回升,58 d略微下降;在2和44 d时的表达丰度显著高于30 d(P<0.05).以上结果表明:从肉鸡肠道近端CDX2mRNA的表达丰度高于远端(P>0.05).AA肉鸡十二指肠及空肠CDX2 mRNA的表达具有相同的发育模式,表明CDX2 mKNA表达受到发育阶段的调控,且在十二指肠和空肠间具有稳定性. |
| 关 键 词： | 肉鸡 肠道 组织特异性 发育性表达 |
Regional and Ontogenetic Expression of CDX2 mRNA in Intestine of Chicken |
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| ZHAN Xun,FENG You,WANG Xiu-qi,SHU Gang,JIANG Qing-yan,FENG Ding-yuan,SHU Xu-gang.Regional and Ontogenetic Expression of CDX2 mRNA in Intestine of Chicken[J].Journal of South China Agricultural University,2009,30(2). | |
| Authors: | ZHAN Xun FENG You WANG Xiu-qi SHU Gang JIANG Qing-yan FENG Ding-yuan SHU Xu-gang |
| Institution: | 1 Zengcheng Center of CHIATAI Broiler Chicken;South China Agricultural University;Guangzhou 510642;China;2 College of Animal Science;3 Guangzhou Tianke Co.Ltd.;Guangzhou 510627;China |
| Abstract: | One hundred and twenty 1-day-old parental male Arbor Acre(AA) broiler chicks were randomly divided into 4 replicates,respectively.Regional expression of CDX2 mRNA in different segments of intestinal tract of AA chicken on 30 d and ontogenetic expression of CDX2 mRNA in duodenum and jejunum of AA broiler chickens were determined by relative quantitative RT-PCR.It was found that: The CDX2 mRNA expression in duodenum was higher than that in jejunum(P=0.09),ileum(P=0.06) and colorectum(P=0.05),respectively.Onto... |
| Keywords: | CDX2 mRNA |
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