喷施亚油酸对烟草胁迫抗性的影响研究

为探讨亚油酸能否提高烟草的胁迫抗性,检测了不同浓度的亚油酸喷施后,烟草叶片中抗氧化酶活性,活性氧、MDA、渗透调节物质的含量,以及抗逆和抗病相关基因的表达水平。结果显示,亚油酸处理降低了烟叶中的MDA含量,提高了脯氨酸、可溶性糖、H_2O_2含量和POD活性,增强了rbohD、rbohF、WRKY57、PR-1a、JAZ1和APX基因的表达水平,且大多在10 mmol/L时值最高。表明亚油酸能增强烟叶对胁迫的耐受性,且在10 mmol/L亚油酸处理时效果最佳。

Effects of Spraying Linoleic Acid on Stress Resistance of Tobacco

To investigate whether linoleic acid could enhance the stress resistance of tobacco, different concentrations of linoleic acid were sprayed to tobacco. The activities of antioxidant enzymes and the contents of proline, soluble sugar, H2O2 and MDA were detected. The expression levels of genes related to stress resistance were also detected by qPCR. Results showed that linoleic acid reduced the contents of MDA in tobacco leaves, but increased the content of proline, soluble sugar, H2O2 and POD activity. The expression levels of rbohD, rbohF, WRKY57, PR-1a, JAZ1 and APX were also increased, especially at 10 mM concentration. Suggesting that linoleic acid enhanced stress tolerance of tobacco. Moreover, the best effects were got at 10 mM linoleic acid treatment.