| 玉米体细胞胚胎功能基因转化研究 |
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| 引用本文： | 孟繁凡,马宁,赵玲玲,张东琦,朱天奇,单晓辉,苏胜忠,李世鹏,刘宏魁,李贺,吴颖,原亚萍.玉米体细胞胚胎功能基因转化研究[J].玉米科学,2017,25(5):27-31. |
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| 作者姓名： | 孟繁凡 马宁 赵玲玲 张东琦 朱天奇 单晓辉 苏胜忠 李世鹏 刘宏魁 李贺 吴颖 原亚萍 |
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| 作者单位： | 吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062,吉林大学植物科学学院, 长春 130062 |
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| 基金项目： | 国家转基因生物新品种培育重大专项“抗冷转基因玉米新种质新组合培育” （2014ZX0800305B）、吉林省科技厅项目（20130206011NY） |
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| 摘 要： | 22A基因属于TCB1家族,编码GTPase激活因子,与体细胞胚胎的发生密切相关,且只在发育的胚中特异性表达。本研究从玉米体胚中克隆Zm22A基因,并对玉米自交系Y423进行遗传转化。结果表明,以玉米自交系Y423体细胞胚胎为受体,利用Zm22A基因的干扰载体侵染,获得阳性苗。
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| 关 键 词： | 玉米 Zm22A 体细胞胚胎 干扰载体 |
| 收稿时间： | 2017/2/27 0:00:00 |
Transformation Study on the Functional Genes of Maize Somatic Embryos |
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| MENG Fan-fan,MA Ning,ZHAO Ling-ling,ZHANG Dong-qi,ZHU Tian-qi,SHAN Xiao-hui,SU Sheng-zhong,LI Shi-peng,LIU Hong-kui,LI He,WU Ying and YUAN Ya-ping.Transformation Study on the Functional Genes of Maize Somatic Embryos[J].Journal of Maize Sciences,2017,25(5):27-31. |
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| Authors: | MENG Fan-fan MA Ning ZHAO Ling-ling ZHANG Dong-qi ZHU Tian-qi SHAN Xiao-hui SU Sheng-zhong LI Shi-peng LIU Hong-kui LI He WU Ying and YUAN Ya-ping |
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| Institution: | College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China,College of Plant Science, Jilin University, Changchun 130062, China and College of Plant Science, Jilin University, Changchun 130062, China |
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| Abstract: | 22A gene belongs to TCB1 family, coding GTPase activating factor, is closely related to the somatic embryogenesis, and only specific expression in the development of the embryo. Zm22A gene was cloned from the maize somatic embryos, and transferred into maize inbred Y423 by genetic transformation in this study. The results showed that, Somatic Embryos of maize inbred Y423 were trated as explants, the positive plants were obtained that infected by interference vector of Zm22A. |
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| Keywords: | Maize Zm22A Somatic embryos Interference vector |
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