小鼠肝再生过程中脂质代谢相关通路中基因的表达谱变化

[目的]研究肝再生过程中肝脏内脂质代谢通路相关基因的表达谱变化。[方法]建立CC l4诱导肝再生小鼠模型,从小鼠肝组织中提取总RNA,通过微阵列基因芯片技术检测在不同肝再生期间脂质代谢通路相关基因的表达变化,并进行具体功能分析。[结果]肝再生过程中,有98个脂质代谢相关基因的表达水平发生了变化,根据这些基因表达的变化趋势可以分为8组。整体上看,基因表达前期表现为抑制,后期表现为上调。其中,脂肪酸的合成通路基因表达以上调为主,分解代谢通路变化并不明显;大多数胆汁酸合成通路基因在4.5天之前表现为抑制,在4.5天和7天时表现为上调。[结论]肝再生过程中,脂质代谢相关通路间的基因表达变化并不一致,这些数据为进一步研究脂质代谢相关通路对肝再生的调控作用提供了明确的基因范围。

Expression Profile Changes of Genes Involved in Lipid Metabolism Pathway during Liver Regeneration in Mice

[Objective] The aim of the research was to study the expression profile changes of genes involved in lipid metabolism pathway during liver regeneration in mice.[Method] The CCl4 induced mouse model of liver regeneration was established and the total RNA was isolated from liver tissue of mouse.Then the changes of genes involved in lipid metabolism pathway during different stages of liver regeneration were detected through micro-array chip gene technique and their specific functions were also analyzed.[Result] During the process of liver regeneration,the expression level of 98 genes involved in lipid metabolism pathway changed,which were divided into eight groups,accordirg to change trend.In the mass,the expression of genes was inhibited in the early stage and up-regulated in the late phase.And the gene expression associated with fatty acid synthesis pathway was mainly up-regulated while the catabolic pathway did not change significantly.Most of genes involved in bile acid synthesis pathway were suppressed before 4.5 d and up-regulated after 4.5 d or 7 d.[Conclusion] During the process of liver regeneration,the genes associated with lipid metabolism were expressed in different trends,and this data should provide a specific range of genes for further studying the regulation effect of lipid metabolism related pathway on liver regeneration.