Parthenogenetic Induction of Canine Oocytes by Electrical Stimulation and Ca-EDTA |
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| Authors: | SR Lee J-W Kim BS Kim D-H Yoo YS Park T-H Lee J-H Ha B-H Hyun ZY Ryoo |
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| Affiliation: | School of Life Science and Biotechnology, Kyungpook National University, Buk-ku, Daegu, Korea;;Departhment of Oral Biochemistry, The 2nd Stage of BK21 Program for Dental School, Dental Science Research Institute, School of Dentistry, Chonnam National University, Buk-gu, Gwangju, Korea;;Department of Life Science, Buk-gu, Gwangju, Korea;;School of Life Science and Biotechnology, Kyungpook National University, Buk-ku, Daegu, Korea;;Disease Model Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yuseong-gu, Daejeon, Korea |
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| Abstract: | In this study, we investigated parthenogenetic induction of canine oocytes by electrical stimulation following Ca-EDTA treatment. Oocyte maturation, parthenogenetic development, and cleavage rate in canine after various electrical stimulations (1.5, 1.8, 2.1 kV/cm) for 50 μs with single DC pulse following 1 mM Ca-EDTA treatment were investigated. In oocyte activated electrically at the voltage of 1.5 kV/cm after 1 mM Ca-EDTA treatment, the rate of pronucleus and two-cell was 4.1% and 2.7%, respectively. Although electrical stimulation could parthenogenetically induce immature oocyte to cleavage stage, degeneration rate in all experimental groups was more than 60%. This means that electrical stimulation after Ca-EDTA treatment could cause canine oocytes to be degenerated. However, two-cell in canine oocyte by parthenogenesis was for the first time induced. Therefore, we suggested that electrical stimulation for canine oocytes could induce parthenogenetically early embryonic cleavage. This result can be used as a basic data for parthenogenesis study in canine. Also, to perform more developed embryonic development, further study to parthenogenesis in canine need to be developed. |
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