| Production of chitinolytic enzymes with Trichoderma longibrachiatum IMI 92027 in solid substrate fermentation |
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| 作者姓名： | Krisztina Kovacs Gyorgy Szakacs Tunde Pusztahelyi Ashok Pandey |
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| 作者单位： | [1]DepartmentofAgriculturalChemicalTechnology,TechnicalUniversityofBudapest,IIIIBudapest,Gellertter4.,Hungary [2]DepartmentofMicrobiology＆Biotechnology,FacultyofScience,UniversityofDebrecen,4010Debrecen,Egyetemter1,Hungary [3]BiotechnologyDivision,RegionalResearchLaboratory,CSIR,Trivandrum695019,India |
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| 摘 要： | Thirty Trichoderma strains representing 15 species within the genus have been screened for extracellular production of chitinolytic enzymes in solid substrate fermentation (SSF). T.longibrachiatum IMI 92027 (=ATCC 36838) gave the highest yield (5.0 IU/g dry matter of substrate) after 3 days of fermentation on wheat bran-crude chitin (9:1 mixture) medium. The optimum moisture content (66.7%), chitin content (20%), initial pH of the medium (2-5) and time course (5 d) of SSF were determ…
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| 关 键 词： | 固态基质 发酵 酶 木霉属 真菌 |
| 文章编号： | 1008-9209(2004)04-0434-01 |
Production of chitinolytic enzymes with Trichoderma longibrachiatum IMI 92027 in solid substrate fermentation |
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| Krisztina Kovacs,Gyorgy Szakacs,Tunde Pusztahelyi,Ashok Pandey.Production of chitinolytic enzymes with Trichoderma longibrachiatum IMI 92027 in solid substrate fermentation[J].Journal of Zhejiang University(Agriculture & Life Sciences),2004,30(4):434-434. |
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| Authors: | Krisztina Kovacs Gyorgy Szakacs Tunde Pusztahelyi Ashok Pandey |
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| Abstract: | Thirty Trichoderna strains representing 15 species within the genus have been screened for extracellular production of chitinolytic enzymes in solid substrate fermentation (SSF). T.longibrachiatum IMI 92027 ( = ATCC 36838) gave the highest yield (5.0 IU/g dry matter of substrate) after 3 days of fermentation on wheat bran-crude chitin (9:1 mixture) medium. The optimum moisture content (66.7 %), chitin content (20 %), initial pH of the medium (2-5) and time course (5 d) of SSF were determined for strain IMI 92027. No significant effect of different N and P additives was found on the chitinase yield in wheat bran-chitin mixture medium. |
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| Keywords: | Trichoderma longibrachiatum chitinase solid substrate fermentation |
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