转δ-内毒素基因棉与药剂对斜纹夜蛾活性的协同作用

目的]为斜纹夜蛾的综合治理提供科学依据。方法]以转δ-内毒素基因棉(苏抗103)和普通棉花(苏棉12)为材料,比较了两种棉花与4种药剂配合饲喂对斜纹夜蛾生长发育及其对化学药剂敏感性的影响。结果]连续饲养3代后的斜纹夜蛾3龄幼虫对氰戊菊酯、灭多威、敌百虫、Bt可湿粉的敏感性,用苏抗103饲喂的是用苏棉12的0.54、0.610、.80、0.48倍,幼虫用苏抗103浸渍药剂饲喂及用苏棉12浸渍药剂饲喂,前者比后者对药剂的敏感性增强。与取食苏棉12相比,取食苏抗103的斜纹夜蛾幼虫。存活率降低了30%,幼虫期延长了9.6 d,龄数由6龄增加到8龄,且体内乙酰胆碱酯酶及羧酸酯酶的比活力明显提高。结论]苏抗103与化学药剂配合使用后使得斜纹夜蛾的敏感性增强。

Synergistic Effect of Transgenic Cotton with Bt-gene and Insecticide on the Activity of Prodenia litura(Fabrieius)

Objective]The aim of study was to provide scientific basis for the comprehensive control of Prodenia litura.Method]With transgenic cotton with Bt-gene Sukong 103 and common cotton Sumian 12 as tested materials, the effects of combined feeding of 2 cottons with 4 insecticides on the growth and development of P.litura and their insecticide susceptibilities were compared.Result]As for the susceptibilities of 3 instar larva P.litura fed continuously for 3 generations to fenvalerate,methomyl,trichlorfon and Bt WP, the susceptibilities of larva of that fed by Sukong 103 were 0.54,0.61,0.80 and 0.48 times of that fed by Sumian 12.When the larva were fed with Sukong 103 and Sumian 12 resp.immersed insecticide,the susceptibilities of Sukong 103 to insecticide was were more higher than that of Sumian 12.Compared with the larva fed with Sumian 12,the survival rate of the larval fed with Sukong 103 was decreased by 30%,the larval duration was 9.6 days longer,and the instar number increased from 6 instars to 8 instars,the specific activities of acetylcholinesterase and carboxylesterase in larva were obviously higher.Conclusion]Combination of Sukong 103 and chemical insecticide can make susceptibilities of P. litura be stronger.