一株正二十二烷降解菌的分离及鉴定

[目的]研究正二十二烷降解菌的分离及鉴定。[方法]以正二十二烷无机盐培养基为选择培养基,从南充市炼油厂曝气池的回流污泥中筛选出1株高效降解长链烷烃的菌株,命名为T2,并对其进行形态学观察、生理生化鉴定和16S rDNA比对以及17种药物敏感试验。[结果]T2菌株鉴定为沙雷氏菌属。在正二十二烷浓度为1%（W/V）无机盐培养基中接入1%种子液,并在30℃和180 r/min摇瓶震荡下培养6 d,正二十二烷降解率可达70%。药敏试验表明,T2菌株对链霉素、卡拉霉素、大观霉、氯霉素、氧氟沙星、庆大霉素、恩诺沙星、新霉素、阿米卡星、复方新诺明高度敏感;对环丙沙星为中度敏感;对青霉素G、氨苄青霉素、四环素、乙酰螺旋霉素、克林霉素、阿莫西林不敏感。[结论]该研究为石油污染的生物处理提供良好的微生物菌种资源。

Isolation and Identification on A Strain of N-docosane Degrading Bacteria

[Objective] The aim was to study the isolation and identification of n-docosane degrading bacteria.[Method] With the mineral salt medium with the n-docosane as the selecting medium,a strain that could degrade the long-chain alkane effectively was screen out from the return sludge in the aeration pool of Nanchong Oil Refinery Plant,named as T2,and was conducted for the morphological observation,physiological and biochemical identification and 16S rDNA comparison,and the sensitive test with 17 kinds of drugs.[Result] The strain T2 was identified as Serratia sp.The N-docosane degradation was 70% after 1% seed liquid of the strain was inoculated on the mineral salt medium with 1%(W/V) of n-docosane and cultured at 30 ℃ and 180 r/min for 6 d.The drug the sensitivity tests showed that the T2 strain was sensitive to Streptomycin,Kanamycin,Spectinomycin,Chloromycetin,Ofloxacin,Gentamicin,Enrofloxacin,Neomycin,Amikaci and Sulfamethoxazole;intermediately sensitive to Ciprofloxacin;not sensitive to Penicillin G,Ampicillin,Tetracycline,Spiramycin,Clindamycin and Amoxicillin.[Conclusion] The study provided the good resources of the microbial strain for the biological treatment of the petroleum pollution.